School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Center for Tooth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei, Taiwan.
Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan.
J Endod. 2019 Apr;45(4):435-441. doi: 10.1016/j.joen.2018.12.019. Epub 2019 Mar 7.
Although the therapeutic potential of human dental pulp stem cells (hDPSCs) has been studied for bone regeneration, the therapeutic efficiency needs further consideration and examinations for clinical applications. Thus, the aims of this study were to evaluate the effect of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) on the osteogenic differentiation of hDPSCs and to examine the therapeutic efficiency of the THSG-enhanced osseous potential of hDPSCs in alveolar bony defects of rats.
Expressions of osteogenic messenger RNAs (including ALP, RUNX2, BGLAP, and AMBN) were examined by quantitative real-time polymerase chain reaction. Alizarin red S staining was conducted to analyze THSG-induced mineralization of hDPSCs. To investigate the regenerative effects of THSG-treated hDPSCs on dental alveolar bone, bony defects were created in male Sprague-Dawley rats. Defects were treated with Matrigel (Corning Inc, Corning, NY), hDPSCs, or hDPSCs + THSG. After 2 weeks, defect healing was evaluated by micro-computed tomographic and histologic analyses.
In the cell model, THSG induced osteogenesis-associated genes (ALP, RUNX2, and BGLAP) and an enamel-related gene (AMBN), resulting in mineralization as detected by alizarin red S staining after 2 weeks of treatment. In the animal model, THSG increased all parameters of bone formation (the relative bone volume, trabecular thickness, trabecular number, and trabecular separation) in alveolar bony defects of rats. THSG not only improved the quality of newly formed bone but also the quantity of new bone.
These results showed important findings in revealing the THSG-enhanced osteogenic differentiation of hDPSCs and THSG-facilitated bone regeneration, which may provide an alternative option for cell-based regenerative therapy.
尽管人牙髓干细胞（hDPSCs）的治疗潜力已在骨再生方面得到研究，但为了临床应用，其治疗效率仍需进一步考虑和检验。因此，本研究旨在评估 2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（THSG）对 hDPSCs 成骨分化的影响，并检验 THSG 增强 hDPSCs 成骨潜能在大鼠牙槽骨缺损中的治疗效果。
通过实时定量聚合酶链反应（qPCR）检测成骨信使 RNA（包括 ALP、RUNX2、BGLAP 和 AMBN）的表达。茜素红 S 染色分析 THSG 诱导 hDPSCs 矿化的情况。为了研究 THSG 处理的 hDPSCs 对牙牙槽骨的再生效果，在雄性 Sprague-Dawley 大鼠中建立骨缺损模型。使用 Matrigel（康宁公司，康宁，纽约）、hDPSCs 或 hDPSCs+THSG 处理缺损。2 周后，通过 micro-CT 和组织学分析评估缺损愈合情况。
在细胞模型中，THSG 诱导了与成骨相关的基因（ALP、RUNX2 和 BGLAP）和一个牙釉质相关基因（AMBN）的表达，并在 2 周的治疗后通过茜素红 S 染色检测到矿化。在动物模型中，THSG 增加了大鼠牙槽骨缺损中所有骨形成参数（相对骨体积、骨小梁厚度、骨小梁数量和骨小梁分离）。THSG 不仅改善了新形成骨的质量，还增加了新骨的数量。
这些结果在揭示 THSG 增强 hDPSCs 成骨分化和 THSG 促进骨再生方面提供了重要发现，这可能为基于细胞的再生治疗提供了一种替代方案。
