一种新型 HSD3B1 特异性鼠单克隆抗体揭示小鼠睾丸中三种成年 Leydig 细胞群体。
Three populations of adult Leydig cells in mouse testes revealed by a novel mouse HSD3B1-specific rat monoclonal antibody.
机构信息
Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Yamagata, 992-8510, Japan.
Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-8582, Japan; Division of Medical Molecular Cell Biology, Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
出版信息
Biochem Biophys Res Commun. 2019 Apr 16;511(4):916-920. doi: 10.1016/j.bbrc.2019.02.100. Epub 2019 Mar 7.
Leydig cells play a pivotal function in the synthesis of a male sex steroid, testosterone. The ability of the steroid production is dependent on the expression of the steroidogenic genes, such as HSD3B (3β-hydroxysteroid dehydrogenase/Δ5- Δ4 isomerase). It has been established that two different types of Leydig cells, fetal Leydig cells (FLCs) and adult Leydig cells (ALCs), are developed in mammalian testes. FLCs and ALCs are characterized by different sets of marker gene expression. In the case of mouse Leydig cells, Hsd3b1 (Hsd3b type 1) is expressed both in FLCs and ALCs whereas Hsd3b6 (Hsd3b type 6) is expressed in ALCs but not in FLCs. However, because the antibodies established so far for HSD3B were unable to distinguish between the HSD3B1 and HSD3B6 isoforms, it remained unclear whether both of them are expressed in every ALC. Therefore, in the present study, we generated a rat monoclonal antibody specific for mouse HSD3B1. Intriguingly, this monoclonal antibody together with an antibody specific for HSD3B6 identified three populations of ALCs based on the expression levels of these HSD3Bs.
间质细胞在雄性性激素睾酮的合成中起着关键作用。类固醇生成的能力取决于类固醇生成基因的表达，如 HSD3B（3β-羟甾脱氢酶/Δ5-Δ4 异构酶）。已经证实，哺乳动物睾丸中存在两种不同类型的间质细胞，即胎儿间质细胞（FLC）和成年间质细胞（ALC）。FLC 和 ALC 的特征在于不同的标记基因表达谱。在小鼠间质细胞中，Hsd3b1（Hsd3b 类型 1）在 FLC 和 ALC 中均有表达，而 Hsd3b6（Hsd3b 类型 6）仅在 ALC 中表达而不在 FLC 中表达。然而，由于迄今为止为 HSD3B 建立的抗体无法区分 HSD3B1 和 HSD3B6 同工型，因此仍不清楚它们是否都在每个 ALC 中表达。因此，在本研究中，我们生成了一种针对小鼠 HSD3B1 的大鼠单克隆抗体。有趣的是，这种单克隆抗体与针对 HSD3B6 的抗体一起，根据这些 HSD3B 的表达水平，鉴定了三种 ALC 群体。
Zotero 插件