Liang J H, Sankai T, Yoshida T, Cho F, Yoshikawa Y
Tsukuba Primate Center, The National Institute of Infectious Diseases, Ibaraki, Japan.
J Med Primatol. 1998 Feb;27(1):10-4. doi: 10.1111/j.1600-0684.1998.tb00062.x.
The enzyme 3beta-hydroxysteroid dehydrogenase/delta5-delta4-isomerase (3beta-HSD) is essential for the biosynthesis of all classes of steroid hormones, including androgens. We localized testosterone and 3beta-HSD by light microscopic immunocytochemistry in the testes of adult cynomolgus monkeys. Immunoreactive testosterone was located as intense deposits in the labeled cytoplasm of Leydig cells, and located weakly in the interstitial tissues, basement membranes, and the regions near tubular walls within tubules. Immunoreactive 3beta-HSD was located in the cytoplasm of all Sertoli cells and was especially intense in the parts near tubular walls and located weakly to intensely in the cytoplasm of some Leydig cells. This is the first immunocytochemical evidence that Sertoli cells of cynomolgus monkeys, as well as Leydig cells, are involved in biosynthesis of androgens.
3β-羟基类固醇脱氢酶/δ5-δ4异构酶（3β-HSD）对于包括雄激素在内的所有类固醇激素的生物合成至关重要。我们通过光学显微镜免疫细胞化学方法在成年食蟹猴的睾丸中定位了睾酮和3β-HSD。免疫反应性睾酮以密集沉积物的形式位于Leydig细胞的标记细胞质中，在间质组织、基底膜以及小管内靠近管壁的区域中定位较弱。免疫反应性3β-HSD位于所有支持细胞的细胞质中，在靠近管壁的部分尤其强烈，在一些Leydig细胞的细胞质中定位较弱至强烈。这是首次通过免疫细胞化学证据表明食蟹猴的支持细胞以及Leydig细胞参与雄激素的生物合成。
