Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.
Ecotoxicol Environ Saf. 2019 Jun 15;174:429-434. doi: 10.1016/j.ecoenv.2019.03.016. Epub 2019 Mar 7.
Pentachloronitrobenzene (PCNB) has been widely utilized as a fungicide to control diseases. However, toxic effect data of PCNB on terrestrial invertebrate are not available till now. Herein, the earthworms (Eisenia fetida) were exposed to soil containing different levels of PCNB. Mortality, weight, accumulation, and physiological indexes of earthworms were determined on certain days. PCNB inhibited the growth of earthworms and induced a significant increase in the activity of antioxidative enzymes. ROS, SOD, and MDA of earthworms in the highest treatment group were 6.8, 4.4, and 3.8 times higher than those in the control group, respectively. In addition, earthworm coelomocytes were successfully extracted, cultured, and innovatively employed in in-vitro toxicity test to evaluate the toxic effect of PCNB. The biomarkers utilized in in-vitro toxicity test, including cell viability, intracellular ROS and extracellular LDH showed significant correlations with the PCNB in the culture media, indicating that the in-vitro toxicity test may serve as a useful tool for toxic assessment of pollutants to earthworms and other organisms.
五氯硝基苯（PCNB）曾被广泛用作杀菌剂来防治疾病。然而，目前为止，还没有关于 PCNB 对陆生无脊椎动物的毒性效应数据。本研究将赤子爱胜蚓暴露于含有不同浓度 PCNB 的土壤中，在特定天数内测定了死亡率、体重、积累量和蚯蚓的生理指标。PCNB 抑制了蚯蚓的生长，并诱导抗氧化酶活性显著增加。最高处理组蚯蚓体内的 ROS、SOD 和 MDA 分别比对照组高 6.8、4.4 和 3.8 倍。此外，还成功提取、培养了蚯蚓体腔细胞，并创新性地将其用于体外毒性试验，以评估 PCNB 的毒性效应。体外毒性试验中使用的生物标志物，包括细胞活力、细胞内 ROS 和细胞外 LDH 与培养基中的 PCNB 呈显著相关，表明该体外毒性试验可能成为评估 PCNB 等污染物对蚯蚓和其他生物毒性的有用工具。
