Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China; Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
Bioresour Technol. 2019 Jun;282:94-102. doi: 10.1016/j.biortech.2019.02.127. Epub 2019 Mar 1.
This study explored the co-production of fucoxanthin and stearidonic acid from Isochrysis zhangjiangensis by investigating its carbon metabolism under different light intensities. Results showed high light inhibited the synthesis of fucoxanthin and stearidonic acid, while promoted cell growth and enhanced cellular lipid content compared with low light, achieving 2.4 g/L and 28.55%, respectively. Low light accelerated the accumulation of fucoxanthin and stearidonic acid, which obtained 23.29 mg/g and 17.16% (of total fatty acid). In combination with the molecular analysis, low light redirected carbon skeletons into glyceraldehyde-3-phosphate and diverted into carotenoid especially fucoxanthin. While, high light redistributed the skeletons to Malonyl CoA, citrate and α-Ketoglutarate and then oriented into lipid metabolism. The highest fucoxanthin and stearidonic acid productivity was 2.94 mg L d and 4.33 mg L d, respectively, which revealed I. zhanjiangensis is a potential strain for the co-production of fucoxanthin and stearidonic acid.
本研究通过考察不同光照强度下三角褐指藻的碳代谢，探索了其同时生物合成岩藻黄质和二十二碳六烯酸的情况。结果表明，与弱光相比，强光抑制了岩藻黄质和二十二碳六烯酸的合成，同时促进了细胞生长，提高了细胞脂质含量，分别达到 2.4 g/L 和 28.55%。弱光促进了岩藻黄质和二十二碳六烯酸的积累，分别达到 23.29 mg/g 和 17.16%（占总脂肪酸的比例）。结合分子分析，弱光将碳骨架重新定向为 3-磷酸甘油醛，并将其分流至类胡萝卜素，特别是岩藻黄质。而强光则将骨架重新分配为丙二酰辅酶 A、柠檬酸和α-酮戊二酸，然后定向进入脂质代谢。岩藻黄质和二十二碳六烯酸的最高生产力分别为 2.94 mg/L/d 和 4.33 mg/L/d，这表明三角褐指藻是同时生物合成岩藻黄质和二十二碳六烯酸的潜在菌株。
