Florescu Gune Iulia Elena, Burcea Alexandru, Popa Gina Oana, Dudu Andreea, Georgescu Sergiu Emil, Balas Mihaela, Dinescu Sorina, Voicu Sorina, Grecu Iulia, Dediu Lorena, Dinischiotu Anca, Costache Marieta
University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, Bucharest, Romania.
Department of Aquaculture, Environmental Sciences and Cadastre, "Lower Danube" University of Galați, Faculty of Environmental Science and Biotechnology, Galati, Romania.
Acta Biochim Pol. 2019 Mar 10;66(1):47-59. doi: 10.18388/abp.2018_2712.
Acipenser stellatus represents a species of great economical interest due to its roe used for caviar production. Therefore, it has been intensively captured for decades and nowadays, this species is on the verge of extinction. As a consequence, Acipenser stellatus is intensively raised in fish farms. Aquaculture is focused on optimizing the feeding regime of juveniles. The aim of this study was to investigate if Acipenser stellatus can adapt to a starvation/refeeding regime by assessing the effects of this regime on growth performance, oxidative stress biomarkers and heat shock protein (hsp) gene expression in juveniles raised under aquaculture conditions. The juveniles were subjected to two starvation/refeeding regimes: a 7-day starvation period followed by 21 days of refeeding, and a14-day starvation period followed by 21 days of refeeding. The results had shown that the juveniles subjected to 7/21-day starvation/refeeding regime presented a complete compensatory growth, they were able to counteract the oxidative stress by enhancing activities of the antioxidant enzymes and they presented no significant changes in hsp gene expression. In contrast, 14/21-day starvation/refeeding regime negatively influenced growth performance, it induced a high level of oxidative stress that was impossible to counteract and it determined major changes in the hsp gene expression level in the liver of Acipenser stellatus. Thus, Acipenser stellatus seems to be able to adapt only to the 7/21-day starvation/refeeding regime that does not threaten the growth performance and the welfare of juveniles. Therefore, it could be useful to optimize the feeding practice in aquaculture production.
由于星斑鲟的鱼籽可用于生产鱼子酱，它是一种具有重大经济价值的物种。因此，几十年来它一直遭到过度捕捞，如今该物种已濒临灭绝。结果，人们在养鱼场中大量养殖星斑鲟。水产养殖致力于优化幼鱼的投喂方式。本研究的目的是通过评估饥饿/再投喂方式对水产养殖条件下幼鱼生长性能、氧化应激生物标志物和热休克蛋白（hsp）基因表达的影响，来探究星斑鲟是否能适应这种饥饿/再投喂方式。幼鱼被分为两种饥饿/再投喂方式：7天饥饿期后再投喂21天，以及14天饥饿期后再投喂21天。结果表明，经历7/21天饥饿/再投喂方式的幼鱼呈现出完全补偿性生长，它们能够通过增强抗氧化酶的活性来抵抗氧化应激，并且其hsp基因表达没有显著变化。相比之下，14/21天饥饿/再投喂方式对生长性能产生了负面影响，它引发了高水平的氧化应激，而这种氧化应激无法被抵抗，并且它导致了星斑鲟肝脏中hsp基因表达水平的重大变化。因此，星斑鲟似乎仅能适应7/21天饥饿/再投喂方式，这种方式不会威胁幼鱼的生长性能和健康状况。所以，优化水产养殖生产中的投喂实践可能会有所帮助。
