Griffin B D, Micklem L R, McCann M C, James K, Pepper D S
Thromb Haemost. 1986 Feb 28;55(1):40-6.
A panel of 10 murine monoclonal antibodies to procoagulant FVIII has been developed from the fusion of a single spleen. Balb/c mice were injected with a purified preparation of FVIII: Ag, and antibody production in sera and hybrid culture supernatants was monitored using a specific radiometric screening assay. The antibodies all inhibit FVIII clotting activity in normal plasma, and when immobilised on agarose retain their ability to recognise and bind the FVIII procoagulant protein. Studies on protein A-purified immunoglobulins demonstrate a range of properties within the panel of antibodies with regard to species cross-reactivity, clotting inhibition and immunoadsorption. The panel of antibodies has been used to screen heat-treated FVIII concentrates for the occurrence of heat-induced neoantigens.
通过单个脾脏的融合，已开发出一组针对促凝血因子VIII的10种鼠单克隆抗体。将Balb/c小鼠注射纯化的因子VIII：抗原制剂，并使用特定的放射性筛选测定法监测血清和杂交培养上清液中的抗体产生。这些抗体均抑制正常血浆中的因子VIII凝血活性，并且固定在琼脂糖上时仍保留其识别和结合因子VIII促凝血蛋白的能力。对蛋白A纯化的免疫球蛋白的研究表明，该组抗体在物种交叉反应性、凝血抑制和免疫吸附方面具有一系列特性。该组抗体已用于筛选热处理的因子VIII浓缩物中热诱导新抗原的出现情况。
