Miura Ana Carolina, de Barros Luiz Daniel, Ferreira Fernanda Pinto, Neto José Mauricio Ferreira, Sicupira Franco Patricia M L, Su Chunlei, Vidotto Odilon, Garcia João Luis
Protozoology Laboratory, Departament of Preventive Veterinary Medicine, Universidade Estadual de Londrina- UEL- Londrina, Paraná, 86050-970, Brazil.
Department of Microbiology, University of Tennessee, Knoxville, TN, 37996-0845, USA.
Parasitol Res. 2019 May;118(5):1593-1599. doi: 10.1007/s00436-019-06274-1. Epub 2019 Mar 9.
The present study aimed to isolate and genotype strains of T. gondii from pigs slaughtered for human consumption in South Brazil. Blood and tissues (heart, diaphragm, liver, tongue, and masseter) from 400 animals were collected at two slaughterhouses. Sera were obtained, and antibodies against T. gondii were detected by both indirect fluorescence antibody test (IFAT) and modified agglutination test (MAT). The tissues of animals that tested positive in MAT, IFAT, or both (cut-off ≥ 64) were bioassayed. Twenty-six (6.5%) of the 400 animals were positive by serology. A total of 18 (69.2%) out of those 26 were positive in the mouse bioassay. The isolates were characterized by using 10 PCR-RFLP genetic markers. Fourteen isolates were fully genotyped, and four isolates were genotyped using nine of the 10 markers. All isolates belonged to ToxoDB PCR-RFLP genotype #206. The present study reports on genotype #206 in pigs for the first time, and it confirms the atypical nature of the Brazilian T. gondii isolates. Additionally, even with low levels of antibodies detected in pig herds, pork presents a T. gondii infection risk for humans.
本研究旨在从巴西南部供人类食用而屠宰的猪中分离出刚地弓形虫菌株并进行基因分型。在两家屠宰场采集了400头动物的血液和组织（心脏、横膈膜、肝脏、舌头和咬肌）。获取血清，并通过间接荧光抗体试验（IFAT）和改良凝集试验（MAT）检测抗刚地弓形虫抗体。对在MAT、IFAT或两者中检测呈阳性（临界值≥64）的动物组织进行生物测定。400头动物中有26头（6.5%）血清学检测呈阳性。在这26头动物中，共有18头（69.2%）在小鼠生物测定中呈阳性。使用10个PCR-RFLP基因标记对分离株进行鉴定。14个分离株进行了完全基因分型，4个分离株使用10个标记中的9个进行了基因分型。所有分离株均属于ToxoDB PCR-RFLP基因型#206。本研究首次报道了猪中的#206基因型，并证实了巴西刚地弓形虫分离株的非典型性质。此外，即使在猪群中检测到的抗体水平较低，猪肉对人类仍存在刚地弓形虫感染风险。
