Muniz F L, Ximenes A M, Bittencourt P S, Hernández-Rangel S M, Campos Z, Hrbek T, Farias I P
Laboratory of Animal Genetics and Evolution (LEGAL), Department of Genetics, Federal University of Amazonas (UFAM), Manaus, Amazonas, Brazil.
Graduate Program in Genetics, Conservation and Evolutionary Biology, National Institute for Amazonian Research (INPA), Manaus, Amazonas, Brazil.
Mol Biol Rep. 2019 Apr;46(2):2473-2484. doi: 10.1007/s11033-019-04709-7. Epub 2019 Mar 9.
We isolated and characterized 10 new microsatellites loci for Paleosuchus trigonatus using ION TORRENT Sequencing Technology. We tested the transferability of these loci to three related species of the subfamily Caimaninae, and used these bi-parental markers to test population structure and genetic diversity of two populations of P. trigonatus impacted by hydroelectric dam construction on the Madeira (N = 16) and Xingu (N = 16) rivers. We also investigated the transferability of these markers to three related species: Paleosuchus palpebrosus (N = 5), Caiman crocodilus (N = 6) and Melanosuchus niger (N = 6). The genetic diversity of P. trigonatus was low in both the Madeira (He: 0.535 ± 0.148) and Xingu (He: 0.381 ± 0.222) populations, but the loci were sufficiently polymorphic to be used in system of mating and kinship studies in P. trigonatus. DAPC analysis with our set of microsatellites loci was able to separate the four species of Caimaninae studied and to detect a shallow genetic structure between Madeira and Xingu populations of P. trigonatus. AMOVA and STRUCTURE analyses using locprior model corroborate this shallow genetic structure. These novel molecular markers will be also useful in conservation genetics and phylogeographic studies of P. trigonatus, since they improve our ability to monitor the putative effects of dams on the loss of genetic diversity and allow us to investigate population dynamics and microevolutionary processes that occurred in the species.
我们使用ION TORRENT测序技术分离并鉴定了10个新的三角古鳄微卫星位点。我们测试了这些位点在凯门鳄亚科三个相关物种中的转移性，并使用这些双亲标记来测试受马德拉河（N = 16）和欣古河（N = 16）上水电大坝建设影响的两个三角古鳄种群的种群结构和遗传多样性。我们还研究了这些标记在三个相关物种中的转移性：睑古鳄（N = 5）、眼镜凯门鳄（N = 6）和黑凯门鳄（N = 6）。三角古鳄在马德拉河（He：0.535±0.148）和欣古河（He：0.381±0.222）种群中的遗传多样性都较低，但这些位点具有足够的多态性，可用于三角古鳄的交配系统和亲属关系研究。使用我们的微卫星位点集进行的DAPC分析能够区分所研究的凯门鳄亚科的四个物种，并检测到三角古鳄马德拉河和欣古河种群之间的浅层遗传结构。使用locprior模型的AMOVA和STRUCTURE分析证实了这种浅层遗传结构。这些新的分子标记在三角古鳄的保护遗传学和系统地理学研究中也将有用，因为它们提高了我们监测大坝对遗传多样性丧失的假定影响的能力，并使我们能够研究该物种中发生的种群动态和微进化过程。
