The Solomon H. Snyder Department of Neuroscience and the Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
The Solomon H. Snyder Department of Neuroscience and the Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Exp Neurol. 2019 Jul;317:129-132. doi: 10.1016/j.expneurol.2019.03.002. Epub 2019 Mar 7.
The continuous advancement of microscopic imaging techniques combined with the discovery and use of more powerful calcium indicators has made calcium imaging technology much more effective and has increased its use in the study of pain circuitry. Using calcium imaging to study spinal pain mechanisms causes less damage to animals compared to electrophysiological techniques and is also able to observe the firing pattern of spinal neurons and the connections between them on a large scale. These advantages allow any changes in spinal cord circuits caused by pain transmission to be observed more effectively. This review will discuss the development of calcium indicators over the past decades as well as the various applications of calcium imaging, from in vitro to in vivo spinal cord experiments, in the study of pain circuits. We will also discuss possible directions for the study of spinal pain circuits in the future.
随着显微镜成像技术的不断进步，以及更强大的钙指示剂的发现和应用，钙成像技术变得更加有效，并在疼痛回路的研究中得到了更广泛的应用。与电生理技术相比，使用钙成像技术研究脊髓疼痛机制对动物的损伤较小，同时还能够大规模观察脊髓神经元的发射模式及其之间的连接。这些优势使得能够更有效地观察疼痛传递引起的脊髓回路的任何变化。本综述将讨论过去几十年钙指示剂的发展以及钙成像在从体外到体内脊髓实验等各个方面在疼痛回路研究中的应用。我们还将讨论未来研究脊髓疼痛回路的可能方向。
