Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan; Japan Agency for Medical Research and Development, CREST, Tokyo, Japan.
Free Radic Biol Med. 2019 Nov 20;144:218-222. doi: 10.1016/j.freeradbiomed.2019.02.034. Epub 2019 Mar 7.
Human individuals are continually exposed to various exogenous and endogenous reactive electrophiles, which readily react with nucleophilic biomacromolecules, such as protein, and form a variety of covalent adducts. The covalent modifications of protein are thought to be involved in various physiological and pathological processes. Recently, the "adductome", a new concept that represents the totality of covalent adducts bound to nucleophilic biomolecules, has been offered as a useful technique for characterizing essentially all reactive electrophilic compounds in biological samples. The primary advantage of this approach is that non-targeted comprehensive analysis can readily be extended to investigate covalent adduct pattern of different situation of exposure and thereby makes it possible to detect/identify not only known but also unknown adducts. In this review, we provide a summary of the concept and methodology of protein adductomics, especially focusing on redox protein adductomics.
人体个体不断暴露于各种外源性和内源性的亲电试剂中，这些亲电试剂容易与亲核生物大分子（如蛋白质）发生反应，形成各种共价加合物。蛋白质的共价修饰被认为与各种生理和病理过程有关。最近，“加合物组学”作为一种有用的技术，提出了一个新概念，代表了与亲核生物大分子结合的全部共价加合物，用于表征生物样品中基本上所有的亲电反应性化合物。这种方法的主要优点是，非靶向的综合分析可以很容易地扩展到研究不同暴露情况下的共价加合物模式，从而有可能检测/识别不仅是已知的，而且是未知的加合物。在这篇综述中，我们提供了蛋白质加合物组学的概念和方法学的总结，特别是侧重于氧化还原蛋白质加合物组学。
