溴脱氧尿苷诱导脑卒中大鼠过度活跃。
BrdU-induced hyperlocomotion in the stroked rat.
机构信息
Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, United States.
Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, United States; Center for Neurological Restoration, Department of Neurosurgery, Neurological Institute, Cleveland Clinic, Cleveland, OH 44195, United States.
出版信息
Neurosci Lett. 2019 Jun 11;703:96-98. doi: 10.1016/j.neulet.2019.03.007. Epub 2019 Mar 7.
5-bromo-2'-dexoyuridine (BrdU) is often used in neuroscience research as a marker of newly-divided cells. However, several studies suggest that BrdU can produce unwanted side effects, including changes in animal behavior and cellular function. In this study, we investigated the effect of BrdU injections on locomotor behavior in a rodent model of ischemic stroke. Ischemic strokes were induced in adult rats, and 50 mg/kg BrdU was intraperitoneally injected over 5 days beginning 2 weeks post-stroke, while control animals received vehicle. Locomotor activity was evaluated by videotaping the rats in their home cages for 30 min, beginning one hour after BrdU injection. BrdU-injected rats showed a nearly three-fold increase in locomotor activity compared to control animals. These findings suggest that BrdU induces a hyperlocomotor effect in rats following brain injury, pointing to the need for caution when interpreting behavioral results in such studies.
5-溴-2'-脱氧尿苷（BrdU）常用于神经科学研究，作为新分裂细胞的标志物。然而，几项研究表明 BrdU 可能产生不必要的副作用，包括动物行为和细胞功能的改变。在这项研究中，我们调查了 BrdU 注射对缺血性中风啮齿动物模型中运动行为的影响。成年大鼠诱导缺血性中风，BrdU 以 50mg/kg 的剂量腹腔注射 5 天，从中风后 2 周开始，而对照组动物给予载体。通过在 BrdU 注射后 1 小时在大鼠的笼中录像 30 分钟来评估运动活动。与对照组动物相比，BrdU 注射的大鼠的运动活性增加了近三倍。这些发现表明，BrdU 在脑损伤后诱导大鼠的过度运动效应，这表明在这类研究中解释行为结果时需要谨慎。
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