佛波酯与A23187协同刺激培养的肾小管细胞中的磷酸盐蓄积。

Phorbol esters stimulate phosphate accumulation synergistically with A23187 in cultured renal tubular cells.

作者信息

Kinoshita Y, Fukase M, Yamatani T, Hishikawa R, Fujita T

出版信息

Biochem Biophys Res Commun. 1986 Apr 14;136(1):177-82. doi: 10.1016/0006-291x(86)90892-2.

Abstract

The effects of phorbol esters and diacylglycerol on phosphate accumulation in the cultured mouse kidney cells were investigated to assess the possible role of Ca2+-activated, phospholipid dependent protein kinase (protein kinase C) on the renal phosphate handling. 12-O-tetradecanoyl phorbol-13-acetate (TPA) stimulated phosphate accumulation dose-dependently. TPA-induced phosphate accumulation was synergistically enhanced with A23187. 4 alpha-phorbol 12,13-didecanoate did not stimulate the phosphate accumulation, while 4 beta-phorbol 12,13-didecanoate stimulated it. Additionally, 1-oleoyl-2-acetyl-glycerol exhibited a stimulatory effect on phosphate accumulation. These data indicated that protein kinase C is one of possible regulators of phosphate transport at the renal tubules.

摘要

研究了佛波酯和二酰基甘油对培养的小鼠肾细胞中磷酸盐积累的影响，以评估Ca2+激活的磷脂依赖性蛋白激酶（蛋白激酶C）在肾脏磷酸盐处理中的可能作用。12-O-十四酰佛波醇-13-乙酸酯（TPA）剂量依赖性地刺激磷酸盐积累。TPA诱导的磷酸盐积累与A23187协同增强。4α-佛波醇12,13-二癸酸酯不刺激磷酸盐积累，而4β-佛波醇12,13-二癸酸酯刺激磷酸盐积累。此外，1-油酰基-2-乙酰基甘油对磷酸盐积累有刺激作用。这些数据表明蛋白激酶C是肾小管磷酸盐转运的可能调节因子之一。