Groves R L, Kennedy G G, Walgenbach J F, Moyer J W
Department of Entomology, North Carolina State University, Raleigh 27695.
Department of Plant Pathology, North Carolina State University, Raleigh 27695.
Plant Dis. 1998 Aug;82(8):959. doi: 10.1094/PDIS.1998.82.8.959B.
Tomato spotted wilt virus (TSWV) is an economically important virus of many crops throughout the world. Cotton, Gossypium hirsutum L., has previously been demonstrated to be susceptible to TSWV (1). During the fall of 1996, cotton was assayed as a potential host of TSWV, as it is an important early season host of thrips vectors of TSWV. Four commercial cotton varieties (DP 20, DP 52, DP 5409, and HS 46) were screened for susceptibility to four isolates of TSWV: two from tobacco, one from pineapple, and one from dahlia. Greenhouse-grown plants in the first true leaf stage were inoculated mechanically. Mean percent infection ranged from 20 to 33% (x = 27%) across all cotton varieties screened against all virus isolates 21 days post-inoculation. TSWV was recovered through mechanical inoculation from double antibody sandwich-enzyme-linked immunosorbent assay (DAS ELISA) positive cotton leaves in 83% of indicator plants, Emilia sonchifolia (L.) DC. ex Wight. Individual cotton plants testing TSWV ELISA positive were held for an additional 28 days with 4% of these again testing virus positive. The four cotton varieties were tested in the cotyledon, one-leaf, and four-leaf stages for susceptibility to tobacco thrips, Frankliniella fusca Hinds, transmission of one TSWV isolate from tobacco. First instar thrips were obtained from an avirulent colony. Mean percent infection among all varieties assayed was 10, 63, and 5%, respectively, for the three developmental stages. Cotton appears to be most susceptible in the one-leaf developmental stage with potential to serve as a source for TSWV spread to other crops. Reference: (1) G. L. Schuster and R. S. Haliwell. Plant Dis. 78:100, 1994.
番茄斑萎病毒（TSWV）是一种在全球对许多作物具有重要经济影响的病毒。棉花（陆地棉）先前已被证明对TSWV敏感（1）。在1996年秋季，对棉花作为TSWV的潜在寄主进行了检测，因为它是TSWV蓟马传播媒介的重要早期寄主。对四个商业棉花品种（DP 20、DP 52、DP 5409和HS 46）进行了对四种TSWV分离株敏感性的筛选：两种来自烟草，一种来自菠萝，一种来自大丽花。对处于第一片真叶期的温室种植植株进行机械接种。接种21天后，在针对所有病毒分离株筛选的所有棉花品种中，平均感染率在20%至33%之间（x = 27%）。通过机械接种，在83%的指示植物（一点红）中从双抗体夹心酶联免疫吸附测定（DAS ELISA）呈阳性的棉花叶片中分离到了TSWV。对TSWV ELISA呈阳性的单个棉花植株再保留28天，其中4%再次检测呈病毒阳性。对四个棉花品种在子叶期、一叶期和四叶期进行了对烟草蓟马（西花蓟马）传播一种来自烟草的TSWV分离株的敏感性测试。一龄蓟马取自无毒菌落。在测定的所有品种中，三个发育阶段的平均感染率分别为10%、63%和5%。棉花在一叶发育阶段似乎最易感，有可能成为TSWV传播到其他作物的源头。参考文献：（1）G. L. Schuster和R. S. Haliwell。植物病害。78:100，1994。
