Department of Environmental Science, Hankuk University of Foreign Studies, 81 Oedae-ro, Mohyeon-myeon, Cheoin-gu, Youngin-si, Gyeonggi-do 17035, Republic of Korea.
Department of Environmental Science, Hankuk University of Foreign Studies, 81 Oedae-ro, Mohyeon-myeon, Cheoin-gu, Youngin-si, Gyeonggi-do 17035, Republic of Korea.
Sci Total Environ. 2018 Jun 15;627:1174-1181. doi: 10.1016/j.scitotenv.2018.01.243. Epub 2018 Feb 6.
This study investigated the changes in the indigenous microbial community structure with hemoglobin (Hb) application to determine the role of Hb in Hb-based remediation of crude oil-contaminated soil. The phylogenetic diversity of the bacterial community showed that the Hb addition selected surfactants-producing species, thereby, promoting TPH degradation. The significant increase in the CO generation, which can be related to the increase in the bacterial abundance inferred from the 16S rRNA gene copy number, supports the enhanced TPH degradation with Hb application. The similar residual TPH concentrations in the presence of only hydrogen peroxide (HO) and both Hb and HO suggested that the role of Hb as a catalyst was not as significant as the role of Hb as a nutrient. Also, in the presence of HO, a greater recovery of the microbial community structure was observed with the double Hb injection than the single Hb injection. Overall, this study shows that the Hb-based remediation strategies via microbial metabolism can be successfully applied to remediate the crude-oil contaminated Kuwaiti soil.
本研究调查了血红蛋白（Hb）应用过程中土著微生物群落结构的变化，以确定 Hb 在基于 Hb 的原油污染土壤修复中的作用。细菌群落的系统发育多样性表明，Hb 的添加选择了产生表面活性剂的物种，从而促进了 TPH 的降解。CO 的生成显著增加，这可以与从 16S rRNA 基因拷贝数推断出的细菌丰度的增加有关，支持了 Hb 应用对 TPH 降解的增强。仅存在过氧化氢（HO）和 Hb 和 HO 时的残余 TPH 浓度相似，这表明 Hb 作为催化剂的作用不如 Hb 作为营养物的作用重要。此外，在存在 HO 的情况下，与单次 Hb 注射相比，双次 Hb 注射后微生物群落结构的恢复更大。总的来说，本研究表明，通过微生物代谢的基于 Hb 的修复策略可以成功应用于修复科威特受原油污染的土壤。
