College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China.
Product R & D,Lanzhou Weitesen Biological Technology Co. Ltd., Lanzhou, 730030, China.
Microb Pathog. 2019 May;130:137-145. doi: 10.1016/j.micpath.2019.02.035. Epub 2019 Mar 9.
The silkworm (Bombyx mori) is a typical and economically important lepidopteran species, and research has resulted in the development and accumulation of breeding lines. Studies of immune-related silkworm genes not only promote our understanding of silkworm immune response mechanisms, but they also inform insect immune molecular diversity research. Here, silkworm proteins were screened using proteomics after Bombyx mori nuclear polyhedrosis virus (BmNPV) infection, and 2368 silkworm proteins were identified, including six antimicrobial peptides and 12 serpins. The mRNA expression levels of these 18 proteins were examined at different times. The results indicated that attacin had the highest expression level, while serpin-5 and cecropin-D exhibited a negative regulatory correlation. These results provide a significant step toward a deeper understanding of B. mori immunoregulation.
家蚕（Bombyx mori）是一种典型的、具有重要经济意义的鳞翅目昆虫，其培育品系的发展和积累得益于相关研究。免疫相关家蚕基因的研究不仅促进了我们对家蚕免疫反应机制的理解，还为昆虫免疫分子多样性研究提供了信息。本研究采用蛋白质组学方法筛选了感染家蚕核型多角体病毒（BmNPV）后的家蚕蛋白，共鉴定出 2368 种家蚕蛋白，包括 6 种抗菌肽和 12 种丝氨酸蛋白酶抑制剂。进一步检测了这 18 种蛋白在不同时间的 mRNA 表达水平。结果表明，抗菌肽 attacin 的表达水平最高，而丝氨酸蛋白酶抑制剂-5 和 cecropin-D 呈负调控相关。这些结果为深入了解家蚕免疫调控机制迈出了重要一步。
