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[通过定量显微分光荧光测定法证明氧化酶细胞内活性的异质性:两种细胞株的比较]

[Demonstration of the heterogeneity of the intracellular activity of oxidases by quantitative microspectrofluorimetry: comparison of two cell strains].

作者信息

Anthelme B, Lautier D, Salmon J M, Viallet P

出版信息

C R Acad Sci III. 1986;302(9):325-30.

PMID:3085885
Abstract

A microspectrofluorimeter has been used for kinetic studies of the decrease of polycyclic aromatic hydrocarbons (PAH) fluorescence in single living cells (3T3 and RTG2 fibroblasts). These studies allow the determination of activation rate constants (k) for PAH. The distribution of the cellular populations has been represented as a function of k. The histograms show: an heterogeneity of the cellular population; differences between the metabolic activities of the two strains; modifications of the metabolization depending upon the probe tested.

摘要

一台显微分光荧光计已用于对单个活细胞(3T3和RTG2成纤维细胞)中多环芳烃(PAH)荧光衰减的动力学研究。这些研究能够测定PAH的活化速率常数(k)。细胞群体的分布已表示为k的函数。直方图显示:细胞群体的异质性;两种菌株代谢活性之间的差异;代谢作用随所测试探针的不同而发生的变化。

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