Ram Babu, Fartyal Dhirendra, Sheri Vijay, Varakumar Panditi, Borphukan Bhabesh, James Donald, Yadav Renu, Bhatt Arun, Agrawal Pawan K, Achary V Mohan M, Reddy Malireddy K
Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Department of Biotechnology, Uttarakhand Technical University, Dehradun, India.
Front Plant Sci. 2019 Feb 25;10:37. doi: 10.3389/fpls.2019.00037. eCollection 2019.
Fertilizers and herbicides are two major components in the agriculture system for achieving crop productivity. Massive use of orthophosphate fertilizers and herbicides poses threats to phosphate reserves and aids the evolution of herbicide tolerant weed biotypes. Phosphite (Phi), a phosphate analog, has been proposed as more beneficial than traditionally used phosphate fertilizers and herbicides in the agriculture. We developed overexpressing transgenic rice that minimizes the phosphate loss and contributes to weed management in the agriculture. The rice lines showed improved root, shoot length and total biomass production under phosphite conditions. Additionally, the complete phenotype and productivity of lines under the phosphite treatment attained was similar to that of plants under phosphate sufficient condition. The Phi metabolizing properties of the overexpressed lines improved under the Phi application and phi treatment enabled controlling of weeds without compromising the yield of transgenic rice plants. Our results indicated that alone or in combination with other Phi metabolizing gene(s) can possibly be used as an effective ameliorating system for improving crop plants for phi-based fertilization and weed management strategy in the agriculture.
肥料和除草剂是农业系统中实现作物高产的两个主要组成部分。大量使用正磷酸盐肥料和除草剂对磷储备构成威胁，并助长了耐除草剂杂草生物型的进化。亚磷酸盐（Phi）是一种磷酸盐类似物，在农业中被认为比传统使用的磷酸盐肥料和除草剂更有益。我们培育了过表达转基因水稻，可最大限度地减少磷损失，并有助于农业中的杂草管理。这些水稻品系在亚磷酸盐条件下根系、地上部长度和总生物量产量均有所提高。此外，亚磷酸盐处理下这些品系的完整表型和生产力与磷充足条件下的植株相似。过表达品系的Phi代谢特性在施用Phi时得到改善，Phi处理能够控制杂草，同时不影响转基因水稻植株的产量。我们的结果表明，单独或与其他Phi代谢基因组合，Phi可能用作一种有效的改良系统，以改善作物，用于基于Phi的农业施肥和杂草管理策略。
