他莫昔芬对小鼠DNA完整性的影响。
Effects of Tamoxifen on DNA Integrity in Mice.
作者信息
Sadeghi Sepideh, Talebi Ali Reza, Shahedi Abbas, Moein Mohammad Reza, Abbasi-Sarcheshmeh Abolghasem
机构信息
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
出版信息
J Reprod Infertil. 2019 Jan-Mar;20(1):10-15.
BACKGROUND
Tamoxifen (TX) is widely used to treat idiopathic infertility in men. Using TX has been shown to produce sperm in patients with oligospermia and azoospermia and improve male fertility. The aim of this study was to evaluate the effects of TX on DNA and chromatin quality in mice regarding the importance of chromatin quality and sperm DNA at all stages of reproduction.
METHODS
24 male NMRI mice were divided into 3 groups including dose 0.4 that received basal diet and TX, dose 0.6 that received basal diet and TX, and group 3 that received vehicle for 35 days as the control. After that, epididymal spermatozoa were analyzed for nuclear DNA quality One-way ANOVA was performed with a Tukey test to compare sperm DNA fragmentation at different times. The p<0.05 was considered significant.
RESULTS
The use of different doses of TX may have detrimental effects on sperm chromatin protamination and DNA integrity in mice. According to Acridine Orange (AO) staining, the rate of increased single-stranded DNA damage was observed at 0/6 TX dose (p<0.05).
CONCLUSION
The use of different studied TX doses in the animal sample was found to increase the amount of protamine deficiency and DNA defect in treated mice.
背景
他莫昔芬（TX）被广泛用于治疗男性特发性不育症。已证明使用TX可使少精子症和无精子症患者产生精子，并提高男性生育能力。本研究的目的是评估TX对小鼠DNA和染色质质量的影响，因为染色质质量和精子DNA在生殖的各个阶段都很重要。
方法
将24只雄性NMRI小鼠分为3组，包括接受基础饮食和TX的0.4剂量组、接受基础饮食和TX的0.6剂量组，以及接受赋形剂35天作为对照的第3组。之后，对附睾精子的核DNA质量进行分析，采用单因素方差分析和Tukey检验比较不同时间的精子DNA片段化情况。p<0.05被认为具有显著性。
结果
使用不同剂量的TX可能对小鼠精子染色质精蛋白化和DNA完整性产生有害影响。根据吖啶橙（AO）染色，在0/6 TX剂量下观察到单链DNA损伤增加率（p<0.05）。
结论
发现在动物样本中使用不同研究剂量的TX会增加治疗小鼠中鱼精蛋白缺乏和DNA缺陷的量。
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