Griffith T M, Edwards D H, Newby A C, Lewis M J, Henderson A H
Cardiovasc Res. 1986 Jan;20(1):7-12. doi: 10.1093/cvr/20.1.7.
The production (synthesis or release or both) of endothelium derived relaxant factor was studied in rabbit aortic strip preparations and an aortic-coronary artery bioassay system. Production of endothelium derived relaxant factor was rapidly inhibited by agents that inhibit mitochondrial electron transport or F1-ATPase, or which uncouple oxidative phosphorylation, but was only slowly impaired by inhibition of glycolysis. It was dependent also on the presence of extracellular calcium with a rapid on-off response time. This study shows that production of endothelium derived relaxant factor appears to be dependent on both oxidative phosphorylation and extracellular calcium.
在兔主动脉条制备物和主动脉-冠状动脉生物测定系统中，对内皮源性舒张因子的产生（合成或释放或两者兼有）进行了研究。抑制线粒体电子传递或F1-ATP酶的试剂，或使氧化磷酸化解偶联的试剂，可迅速抑制内皮源性舒张因子的产生，但抑制糖酵解时，其产生仅缓慢受损。它还依赖于细胞外钙的存在，且具有快速的开启-关闭反应时间。本研究表明，内皮源性舒张因子的产生似乎依赖于氧化磷酸化和细胞外钙两者。
