Tsuboi K K, Kwong L K, Fan Q, Thompson D J, D'Harlingue A E, Sunshine P
Cell Biochem Funct. 1986 Apr;4(2):131-42. doi: 10.1002/cbf.290040209.
Hydrocortisone administration to infant rats enhanced cellobiase and maltase activities and induced precocious expression of sucrase and trehalase activities along the length of the small intestine. These activity changes reflected proportional concentration increases in the enzymes lactase (EC 3.2.1.23), maltase/glucoamylase (EC 3.2.1.20) and sucrase-isomaltase (EC 3.2.1.48/10). Administration of an equivalent tracer dose of [3H]leucine (by body weight) to control and hydrocortisone-treated infant rats resulted in greater accumulation of label in the carbohydrase pools of the treated rats, suggesting their increased de novo synthesis. The increased concentrations of lactase and maltase/glucoamylase induced by exogenous hydrocortisone were matched by the presence of corresponding greater amounts of label in their brush border pools. Accumulation of label in each of the lactase, maltase/glucoamylase and sucrase-isomaltase pools was generally similar in the hydrocortisone-treated rats, suggesting equivalent stimulation of their synthesis as a group by the humoral agent. The turnover rates of the carbohydrases as a group were found to be similar and did not appear to differ in control and hydrocortisone-treated rats. Total protein synthesis rates were slightly greater in the intestine of the hydrocortisone-treated group of rats.
给幼鼠注射氢化可的松可增强纤维二糖酶和麦芽糖酶的活性，并沿小肠全长诱导蔗糖酶和海藻糖酶活性的早熟表达。这些活性变化反映了乳糖酶（EC 3.2.1.23）、麦芽糖酶/葡糖淀粉酶（EC 3.2.1.20）和蔗糖酶-异麦芽糖酶（EC 3.2.1.48/10）的浓度成比例增加。给对照幼鼠和经氢化可的松处理的幼鼠注射等量示踪剂量的[3H]亮氨酸（按体重）后，处理组大鼠的碳水化合物酶池中标记物的积累更多，表明其从头合成增加。外源性氢化可的松诱导的乳糖酶和麦芽糖酶/葡糖淀粉酶浓度增加，与其刷状缘池中相应更多的标记物存在相匹配。在经氢化可的松处理的大鼠中，乳糖酶、麦芽糖酶/葡糖淀粉酶和蔗糖酶-异麦芽糖酶池中标记物的积累通常相似，表明该体液因子对它们作为一个整体的合成有同等刺激作用。发现碳水化合物酶作为一个整体的周转率相似，在对照大鼠和经氢化可的松处理的大鼠中似乎没有差异。经氢化可的松处理组大鼠小肠中的总蛋白质合成率略高。
