Sugihara H, Kishida M, Nikai T, Azuma H, Yamamoto F, Mori N
Comp Biochem Physiol B. 1986;83(4):743-50. doi: 10.1016/0305-0491(86)90139-2.
Four arginine ester hydrolases, ME-1, 2, 3 and 4 from the venom of Trimeresurus mucrosquamatus had been isolated and characterized by Sugihara et al. (1980, 1981, 1982, 1983). Immunologically, ME-1, 2, 3 and 4 are identical. The four enzymes hydrolyzed Pro-Phe-Arg-MCA and z-Phe-Arg-MCA. Furthermore, ME-2 slightly hydrolyzed Boc-Val-Pro-Arg-MCA, Boc-Phe-Ser-Arg-MCA and Boc-Ile-Glu-Gly-Arg-MCA. ME-1 cleaved almost simultaneously the Arg(22)-Gly(23) and Phe(25)-Tyr(26) bond of oxidized insulin B chain. ME-2 and 3 also hydrolyzed the same bond of insulin B chain, but the activity was not as potent as ME-1. ME-4 did not cleave the substrate. The four enzymes hydrolyzed C-terminal of arginine in the biologically active peptides. Four arginine ester hydrolases showed fibrinogenolytic activity. ME-1 and 2 first cleaved B beta-chain and then A alpha-chain. On the contrary, ME-3 and 4 cleaved A alpha- and B beta-chain simultaneously. The four enzymes also hydrolyzed fibrinogen in plasma cleaving B beta- and gamma-chain and slightly digesting A alpha-chain. The various inhibitors affected TAME (tosyl-arginine-methylester) and the fibrinogen hydrolytic activity of the four enzymes. All four enzymes had fibrinolytic activity.
来自菜花原矛头蝮蛇毒的四种精氨酸酯水解酶，即ME-1、2、3和4，已由杉原等人（1980年、1981年、1982年、1983年）分离并进行了表征。在免疫学上，ME-1、2、3和4是相同的。这四种酶能水解Pro-Phe-Arg-MCA和z-Phe-Arg-MCA。此外，ME-2能轻微水解Boc-Val-Pro-Arg-MCA、Boc-Phe-Ser-Arg-MCA和Boc-Ile-Glu-Gly-Arg-MCA。ME-1几乎同时切割氧化胰岛素B链的Arg(22)-Gly(23)和Phe(25)-Tyr(26)键。ME-2和3也能水解胰岛素B链的相同键，但活性不如ME-1强。ME-4不能切割该底物。这四种酶能水解生物活性肽中精氨酸的C末端。四种精氨酸酯水解酶表现出纤维蛋白原溶解活性。ME-1和2首先切割Bβ链，然后切割Aα链。相反，ME-3和4同时切割Aα链和Bβ链。这四种酶还能水解血浆中的纤维蛋白原，切割Bβ链和γ链，并轻微消化Aα链。各种抑制剂影响TAME（甲苯磺酰精氨酸甲酯）以及这四种酶的纤维蛋白原水解活性。所有四种酶都具有纤维蛋白溶解活性。
