Giannakopoulou Efstathia, Konstantinou Fotios, Ragia Georgia, Gerontitis Zisis, Tavridou Anna, Papapetropoulos Andreas, Mikroulis Dimitrios, Manolopoulos Vangelis G
Laboratory of Pharmacology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece.
Department of Cardiothoracic Surgery, Academic General Hospital of Evros, Alexandroupolis, Greece.
Drug Metab Pers Ther. 2019 Mar 12;34(1):dmpt-2018-0033. doi: 10.1515/dmpt-2018-0033.
Background Cystathionine γ-lyase enzyme, which is encoded by the CTH gene, is responsible for hydrogen sulfide (H2S) production in the endothelium. The CTH 1364 G>T polymorphism may alter the CTH expression and H2S bioavailability, thus leading to atherosclerosis and coronary artery disease (CAD). We examined the potential association of the CTH 1364 G>T polymorphism with CAD. Methods The CTH 1364 G>T polymorphism was determined in 178 coronary artery bypass grafting (CABG) patients and 156 non-atherosclerotic controls of Greek Caucasian origin using the PCR-RFLP method. Results No significant difference in the frequency of the CTH 1364 G>T genotypes (p = 0.281) and alleles (p = 0.265) was found between the CABG patients and controls. After conducting stratification according to sex, analysis showed a numerical difference in the CTH 1364 TT genotype frequency in female participants that did not reach statistical significance (16.3% and 8.5% in the CABG and controls, respectively, p = 0.26). The frequency of the CTH 1364 TT genotype between the male CABG patients and controls did not differ (p = 0.507). Conclusions The CTH 1364 G>T polymorphism was not associated with CAD in the studied population. However, interestingly, a higher - if not significantly so - CTH 1364 TT genotype frequency was present in female CABG patients compared with female controls. Larger studies are necessary to conclude on the potential overall or gender-driven association between CTH 1364 G>T gene polymorphism and CAD.
由CTH基因编码的胱硫醚γ-裂解酶负责在内皮中产生硫化氢（H2S）。CTH 1364 G>T多态性可能会改变CTH表达和H2S生物利用度，从而导致动脉粥样硬化和冠状动脉疾病（CAD）。我们研究了CTH 1364 G>T多态性与CAD之间的潜在关联。方法：采用PCR-RFLP方法，在178例希腊白种人来源的冠状动脉旁路移植术（CABG）患者和156例非动脉粥样硬化对照中测定CTH 1364 G>T多态性。结果：CABG患者和对照之间CTH 1364 G>T基因型频率（p = 0.281）和等位基因频率（p = 0.265）无显著差异。按性别分层后分析显示，女性参与者中CTH 1364 TT基因型频率存在数值差异，但未达到统计学意义（CABG组和对照组分别为16.3%和8.5%，p = 0.26）。男性CABG患者和对照之间CTH 1364 TT基因型频率无差异（p = 0.507）。结论：在研究人群中，CTH 1364 G>T多态性与CAD无关。然而，有趣的是，与女性对照相比，女性CABG患者中CTH 1364 TT基因型频率更高（即使未达到显著水平）。需要更大规模的研究来确定CTH 1364 G>T基因多态性与CAD之间潜在的总体或性别驱动的关联。
