Carey R J
Exp Neurol. 1986 Jun;92(3):591-600. doi: 10.1016/0014-4886(86)90301-8.
Two unilateral injections of 6-hydroxydopamine into the substantia nigra and ventral tegmental area of adult rats pretreated with desmethylimipramine produced severe unilateral depletions of limbic and striatal dopamine. These animals sustained global deficits in spontaneous motor activity with no recovery evident from 1 to 14 weeks postoperative. The frequency of spontaneous circling increased during the 1st few weeks postoperative and then remained stable during the last 2 months of observation. The changes in circling were independent of changes in global spontaneous motor behaviors. Biochemical determinations indicated an increased dopamine turnover in residual limbic and striatal dopamine neurons but with substantially changed dihydroxyphenylacetic acid:homovanillic acid ratios. The absence of functional recovery of both normal and asymmetric motoric behavior indicates that severe uncompensated deficits in motoric function produced by unilateral 6-hydroxydopamine lesions can provide a useful animal model for the study of dopaminergic contributions to movement disorders.
对用去甲丙咪嗪预处理的成年大鼠的黑质和腹侧被盖区进行两次单侧6-羟基多巴胺注射，导致边缘系统和纹状体多巴胺严重单侧耗竭。这些动物在术后1至14周期间持续存在整体自发运动活动缺陷，且无明显恢复迹象。术后最初几周，自发转圈频率增加，然后在观察的最后2个月保持稳定。转圈变化与整体自发运动行为变化无关。生化测定表明，残留边缘系统和纹状体多巴胺神经元中的多巴胺周转率增加，但二羟基苯乙酸：高香草酸比值发生了显著变化。正常和不对称运动行为均无功能恢复，这表明单侧6-羟基多巴胺损伤导致的严重未代偿运动功能缺陷可为研究多巴胺能对运动障碍的影响提供一个有用的动物模型。
