关于鸡胚成纤维细胞胞质DNA复合物大小和组成的进一步研究。

Further studies on the size and composition of the chick embryo fibroblast cytosolic DNA complex.

作者信息

Challen C, Adams D H

出版信息

Int J Biochem. 1986;18(5):423-9. doi: 10.1016/0020-711x(86)90184-9.

Abstract

The chick embryo fibroblast cytosolic DNA complex shows anomalous elution behaviour on agarose gel column chromatography. The indicated molecular size varies between 5 X 10(5) dalton (higher exclusion limit gels) and 1.4 X 10(6) dalton (lower exclusion limit gels). Chromatography on lower exclusion limit gels shows the [3H]thymidine labelled (DNA) complex as a sharp peak, coincident with a peak of [3H]uridine and [3H]lysine labelling and similar pulse labelling patterns for the three precursors but with DNA labelling lagging behind RNA and protein. Both cultured and uncultured cell cytosols show an A260 peak coincident with the [3H]precursor labelling peaks.

摘要

鸡胚成纤维细胞胞质DNA复合物在琼脂糖凝胶柱色谱上呈现异常洗脱行为。所示分子大小在5×10⁵道尔顿（高排阻极限凝胶）和1.4×10⁶道尔顿（低排阻极限凝胶）之间变化。在低排阻极限凝胶上进行色谱分析时，[³H]胸腺嘧啶标记的（DNA）复合物呈现为一个尖锐峰，与[³H]尿苷和[³H]赖氨酸标记峰重合，并且三种前体的脉冲标记模式相似，但DNA标记滞后于RNA和蛋白质。培养和未培养细胞的胞质溶胶均显示出与[³H]前体标记峰重合的A₂₆₀峰。