Turk J Med Sci. 2019 Apr 18;49(2):531-537. doi: 10.3906/sag-1808-130.
BACKGROUND/AIM: This study aimed to comparatively analyze the expression levels of the SLC1A1 gene in renal specimens from tumors and adjacent healthy kidney tissues of patients with clear cell renal cell carcinoma (ccRCC).
Nineteen patients diagnosed with ccRCC were included in the study. The expression levels of the SLC1A1 and GAPDH genes were measured in tumor and formalin-fixed paraffin-embedded (FFPE) tissue specimens from the adjacent healthy kidney of each subject. Via the GEPIA database, the distribution of SLC1A1 gene expressions in ccRCC and healthy kidney tissues was obtained. The relative expression of SLC1A1 was evaluated for the association with the clinical parameters of the patients.
The expression of the SLC1A1 gene was significantly higher in males than females (P = 0.029). Also, there were statistically significant associations between stages II–IV and Fuhrman grades 2–4 with respect to SLC1A1 gene expression (P < 0.001 for both). Moreover, low levels of red blood cell and hemoglobin counts were significantly associated with the SLC1A1 expression (P < 0.001 and P = 0.005, respectively). The expression of the SLC1A1 gene in tumor tissues increased approximately 3 times compared with normal kidney tissues (P < 0.05). According to the GEPIA database, SLC1A1 gene expression is significantly higher in ccRCC patients than healthy persons (P = 0.01).
The change in the expression of SLC1A1 may be crucial for ccRCC pathophysiology.
背景/目的：本研究旨在比较分析透明细胞肾细胞癌（ccRCC）患者肿瘤组织和相邻正常肾组织中 SLC1A1 基因的表达水平。
纳入 19 例经诊断为 ccRCC 的患者。测量每位患者肿瘤和相邻正常肾组织福尔马林固定石蜡包埋（FFPE）组织标本中 SLC1A1 和 GAPDH 基因的表达水平。通过 GEPIA 数据库获得 SLC1A1 基因在 ccRCC 和正常肾组织中的表达分布。评估 SLC1A1 的相对表达与患者临床参数的相关性。
SLC1A1 基因的表达在男性中显著高于女性（P = 0.029）。此外，SLC1A1 基因的表达与 II-IV 期和 Fuhrman 分级 2-4 之间存在统计学显著相关性（均 P < 0.001）。此外，红细胞和血红蛋白计数低与 SLC1A1 表达显著相关（均 P < 0.001 和 P = 0.005）。与正常肾组织相比，肿瘤组织中 SLC1A1 基因的表达增加了约 3 倍（P < 0.05）。根据 GEPIA 数据库，SLC1A1 基因在 ccRCC 患者中的表达明显高于健康人（P = 0.01）。
SLC1A1 表达的变化可能对 ccRCC 发病机制至关重要。
