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乙二胺四乙酸(EDTA)处理后嗜酸性粒细胞颗粒的嗜酸性增强。

Increased acidophilia of eosinophil granules after EDTA treatment.

作者信息

Gomez-Perretta C, Armas-Portela R, Stockert J C, Tato A, Ferrer J M

出版信息

Histochem J. 1986 Jan;18(1):1-4. doi: 10.1007/BF01676191.

Abstract

The acidophilic reaction of eosinophil leucocyte granules from human, pig and horse blood smears was investigated by using May-Grünwald-Giemsa staining after previous treatment with EDTA and sodium citrate solutions. The same peak at 530 nm, but absorption values considerably higher than those of controls, were found in eosinophil granules after application of chelating agents, indicating that removal of metal cations could unmask basic groups in these structures.

摘要

在用乙二胺四乙酸(EDTA)和柠檬酸钠溶液预处理后,采用May-Grünwald-Giemsa染色法,对人、猪和马血涂片嗜酸性粒细胞颗粒的嗜酸性反应进行了研究。在应用螯合剂后,嗜酸性粒细胞颗粒在530nm处出现相同的峰值,但吸收值明显高于对照组,这表明去除金属阳离子可以使这些结构中的碱性基团暴露出来。

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