Fujiwara M, Morikawa S, Taniguchi S, Mori K, Fujiwara M, Takaori S
J Biochem. 1986 Mar;99(3):615-25. doi: 10.1093/oxfordjournals.jbchem.a135520.
Effects of the calmodulin antagonists chlorpromazine, trifluoperazine, and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide on phospholipid metabolism were examined in rabbit platelets using [3H]serine, [3H]ethanolamine, [3H]choline, and [3H]glycerol. All these drugs markedly stimulated the incorporation of [3H]serine into phosphatidylserine. On the other hand, these drugs had only a slight effect on the rate of incorporation of [3H]ethanolamine and [3H]choline into the corresponding phospholipid. When [3H]glycerol was used as a precursor of the phospholipids, 3H-labeled phospholipids were mainly composed of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol. Although the phosphorus content of phosphatidylserine was about 40% of that of phosphatidylcholine in rabbit platelets, the amount of phosphatidylserine labeled with [3H]glycerol was less than 2% of that of the labeled phosphatidylcholine, and calmodulin antagonists slightly stimulated the incorporation of [3H]glycerol into phosphatidylserine. Treatment with calmodulin antagonists caused a marked decrease in the content of endogenous free serine with concomitant increase in the contents of endogenous free ethanolamine and choline. On the other hand, the contents of other free amino acids, including essential and non-essential amino acids, were unchanged. These results suggest that the calmodulin antagonists we used did not affect de novo synthesis of phosphatidylserine, but did stimulate the serine phospholipid base-exchange reaction in rabbit platelets.
使用[3H]丝氨酸、[3H]乙醇胺、[3H]胆碱和[3H]甘油，在兔血小板中研究了钙调蛋白拮抗剂氯丙嗪、三氟拉嗪和N-(6-氨基己基)-5-氯-1-萘磺酰胺对磷脂代谢的影响。所有这些药物均显著刺激了[3H]丝氨酸掺入磷脂酰丝氨酸。另一方面，这些药物对[3H]乙醇胺和[3H]胆碱掺入相应磷脂的速率影响很小。当使用[3H]甘油作为磷脂的前体时，3H标记的磷脂主要由磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰肌醇组成。尽管兔血小板中磷脂酰丝氨酸的磷含量约为磷脂酰胆碱的40%，但用[3H]甘油标记的磷脂酰丝氨酸的量不到标记的磷脂酰胆碱量的2%，且钙调蛋白拮抗剂轻微刺激了[3H]甘油掺入磷脂酰丝氨酸。用钙调蛋白拮抗剂处理导致内源性游离丝氨酸含量显著降低，同时内源性游离乙醇胺和胆碱含量增加。另一方面，包括必需氨基酸和非必需氨基酸在内的其他游离氨基酸的含量未发生变化。这些结果表明，我们使用的钙调蛋白拮抗剂不影响磷脂酰丝氨酸的从头合成，但确实刺激了兔血小板中的丝氨酸磷脂碱基交换反应。
