Zhang Jing, Zhang Zhenyu, Zhang Rui, Zhang Wenfei, Li Haozhe, Li Tianran, Zhang Hongyu, Zheng Weiwei
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Front Physiol. 2019 Feb 26;10:162. doi: 10.3389/fphys.2019.00162. eCollection 2019.
The COP9 signalosome (CSN) is an evolutionarily conserved multi-subunit complex that plays crucial roles in regulating various biological processes in plants, mammals, and the model insect . However, it is poorly studied in non-model insects, whereas its role in fecundity remains unclear. In this study, all nine CSN subunits were identified and characterized in s, a major invasive agricultural tephritid pest. Each subunit gene, except for , encoded a protein containing a PCI/PINT or MPN domain. Phylogenetic analysis revealed that all CSN subunits were individually clustered into a specific branch with their counterparts from other species. All CSN subunit genes were expressed in all detected developmental stages and tissues. Most subunits, except for and , showed the highest expression level in the eggs. Notably, 3 and 5 were significantly enriched in mature female adults. Further analysis of revealed that it was enriched in the ovary and that its ovarian expression level gradually increased with the reproductive development process. RNAi-based knockdown of in female adults significantly reduced the number of laid eggs. The expression level of and , which encode the heterodimer receptors of 20E, and vitellogenin transcripts ( and ) was suppressed in the fat body of female adults injected with dsRNA. Decreased level of Vg1 protein was confirmed by means of Western blots. These data indicate that is involved in female reproduction by regulating 20E signaling and Vg synthesis. Overall, our study may facilitate the development of new strategies for controlling s since it provides insights into the evolution and expression patterns of all CSN subunit genes as well as the critical roles of in female fecundity.
COP9信号体（CSN）是一种进化上保守的多亚基复合体，在调节植物、哺乳动物和模式昆虫的各种生物过程中发挥着关键作用。然而，在非模式昆虫中对其研究较少，其在繁殖力方面的作用仍不清楚。在本研究中，在一种主要的入侵性农业实蝇害虫[具体害虫名称未给出]中鉴定并表征了所有九个CSN亚基。除了[具体亚基名称未给出]外，每个亚基基因都编码一种含有PCI/PINT或MPN结构域的蛋白质。系统发育分析表明，所有CSN亚基都与来自其他物种的对应亚基单独聚类到一个特定分支中。所有CSN亚基基因在所有检测到的发育阶段和组织中均有表达。除了[具体亚基名称未给出]和[具体亚基名称未给出]外，大多数亚基在卵中的表达水平最高。值得注意的是，[亚基3名称]和[亚基5名称]在成熟雌成虫中显著富集。对[具体亚基名称未给出]的进一步分析表明，它在卵巢中富集，并且其卵巢表达水平随着生殖发育过程逐渐增加。在雌成虫中基于RNAi敲低[具体亚基名称未给出]显著减少了产卵数量。在注射dsRNA的雌成虫脂肪体中，编码20E异二聚体受体的[具体基因名称未给出]和[具体基因名称未给出]以及卵黄原蛋白转录本（[具体转录本名称未给出]和[具体转录本名称未给出]）的表达水平受到抑制。通过蛋白质免疫印迹法证实了Vg1蛋白水平降低。这些数据表明，[具体亚基名称未给出]通过调节20E信号传导和Vg合成参与雌虫繁殖。总体而言，我们的研究可能有助于开发控制[具体害虫名称未给出]的新策略，因为它提供了对所有CSN亚基基因的进化和表达模式以及[具体亚基名称未给出]在雌虫繁殖力中的关键作用的见解。
