Beaumelle B D, Vial H J
J Chromatogr. 1986 Mar 28;356(1):187-94. doi: 10.1016/s0021-9673(00)91478-x.
Separation of fatty acid methyl esters and dimethyl acetals from complex biological samples has been achieved by gas-liquid chromatography on a capillary column coated with free fatty acid phase. Response-correcting factors were determined, showing rather large variations with fatty acid length. Polyunsaturated fatty acid methyl esters were shown to have lower responses than saturated species, whereas dimethyl acetals and equivalent methyl esters were found to give similar responses. Total fatty acid and aldehyde compositions of human and simian erythrocytes were determined and compared, showing a somewhat higher level of linoleate and arachidonate, and a lower level of plasmalogens in simian erythrocytes.
通过在涂有游离脂肪酸相的毛细管柱上进行气液色谱法，已实现从复杂生物样品中分离脂肪酸甲酯和二甲基缩醛。测定了响应校正因子，结果表明其随脂肪酸长度变化较大。结果显示，多不饱和脂肪酸甲酯的响应低于饱和脂肪酸甲酯，而二甲基缩醛和相应的甲酯具有相似的响应。测定并比较了人和猿红细胞的总脂肪酸和醛组成，结果表明猿红细胞中亚油酸和花生四烯酸水平略高，而缩醛磷脂水平较低。
