Brown M W, Evans C, Ford J L, Pilling M
J Clin Hosp Pharm. 1986 Apr;11(2):117-23. doi: 10.1111/j.1365-2710.1986.tb00835.x.
The isolation of micro-organisms from the oil-in-water Aqueous Cream BP, has been examined using a variety of solvent systems to disperse the cream prior to membrane filtration or direct inoculation. Pour-plate methods which utilize combinations of either peptone-water (containing 5% w/v polysorbate 80) or nutrient broth (containing 4% w/v Lubrol W) provided the most efficient recovery of Pseudomonas aeruginosa but still allowed less than 20% recovery. White spirit and isopropyl myristate allowed no recovery when used as dispersants. Recoveries of P. aeruginosa varied according to the source of the cream. A combination of 1% w/v polysorbate 80 in 0.1% w/v peptone-water and membrane filtration allowed 63.2% w/v and 67.0% w/v recoveries respectively of Aspergillus niger and Candida albicans from unpreserved aqueous cream, but gave unreproducible results for Escherichia coli and P. aeruginosa. Chlorocresol 0.1% w/v) did not meet the British Pharmacopoeial requirements for efficacy of antimicrobial preservatives when tested against C. albicans using membrane filtration to isolate the micro-organism.
已使用多种溶剂系统对水包油型水性乳膏BP中的微生物进行分离，以便在膜过滤或直接接种之前分散乳膏。倾注平板法使用蛋白胨水（含5%w/v聚山梨酯80）或营养肉汤（含4%w/v月桂醇聚醚）的组合，能最有效地回收铜绿假单胞菌，但回收率仍低于20%。白酒精和肉豆蔻酸异丙酯用作分散剂时无法回收微生物。铜绿假单胞菌的回收率因乳膏来源而异。在0.1%w/v蛋白胨水中加入1%w/v聚山梨酯80并结合膜过滤，从未加防腐剂的水性乳膏中分别回收了63.2%w/v和67.0%w/v的黑曲霉和白色念珠菌，但对大肠杆菌和铜绿假单胞菌的回收结果不可重复。当使用膜过滤分离微生物并针对白色念珠菌进行测试时，0.1%w/v的氯甲酚不符合英国药典对抗菌防腐剂效力的要求。
