Lanier L L, Le A M, Civin C I, Loken M R, Phillips J H
J Immunol. 1986 Jun 15;136(12):4480-6.
We examined the antigenic and functional characteristics of human peripheral blood lymphocytes that differentially express the CD16 (Leu-11) and Leu-19 (NKH-1) antigens. Leu-19 is a approximately 220,000 daltons protein expressed on approximately 15% of freshly isolated peripheral blood lymphocytes. Within the Leu-19+ subset, three distinct populations were identified: CD3-,CD16+,Leu-19+ cells; CD3+,CD16-,Leu-19+ cells; and CD3-,CD16-,Leu-19bright+ cells. Both the CD3+,CD16-,Leu-19+ and CD3-,CD16+,Leu-19+ populations mediated non-major histocompatibility complex (MHC)-restricted cytotoxicity against the NK-sensitive tumor cell K562 and were large granular lymphocytes. CD3-,CD16+,Leu-19+ NK cells were the most abundant (comprising approximately 10% of peripheral blood lymphocytes) and the most efficient cytotoxic effectors. The finding that CD3+,Leu 19+ lymphocytes mediated cytotoxicity against K562 unequivocally demonstrates that a unique subset of non-MHC-restricted cytotoxic CD3+ T lymphocytes are present in the peripheral blood of unprimed, normal individuals. However, CD3+,CD16-,Leu-19+ cells comprised less than 5% of peripheral blood lymphocytes, and the cytotoxic activity of this subset was significantly less than CD3-,CD16+,Leu-19+ NK cells. Most CD3+,Leu-19+ T cells co-expressed the CD2, CD8, and CD5 differentiation antigens. The antigenic and functional phenotype of peripheral blood CD3+,Leu-19+ cytotoxic T lymphocytes corresponds to the interleukin 2-dependent CD3+ cell lines that mediate non-MHC-restricted cytotoxicity against NK-sensitive tumor cell targets. A small population of Leu-19bright+ lymphocytes lacking both CD3 and CD16 was also observed. This population (comprising less than 2% of peripheral blood lymphocytes) contained both large agranular lymphocytes and large granular lymphocytes. CD3-,CD16-,Leu-19bright+ lymphocytes also mediate non-MHC-restricted cytotoxicity. The relationship of these CD3-CD16-,Leu-19bright+ lymphocytes to CD3+ T cells or CD16+ NK cells is unknown.
我们检测了差异表达CD16（Leu-11）和Leu-19（NKH-1）抗原的人外周血淋巴细胞的抗原性和功能特性。Leu-19是一种分子量约为220,000道尔顿的蛋白质，在约15%的新鲜分离外周血淋巴细胞上表达。在Leu-19阳性亚群中，鉴定出三个不同的群体：CD3阴性、CD16阳性、Leu-19阳性细胞；CD3阳性、CD16阴性、Leu-19阳性细胞；以及CD3阴性、CD16阴性、Leu-19高表达阳性细胞。CD3阳性、CD16阴性、Leu-19阳性群体和CD3阴性、CD16阳性、Leu-19阳性群体均介导针对NK敏感肿瘤细胞K562的非主要组织相容性复合体（MHC）限制的细胞毒性，并且都是大颗粒淋巴细胞。CD3阴性、CD16阳性、Leu-19阳性自然杀伤（NK）细胞最为丰富（约占外周血淋巴细胞的10%）且是最有效的细胞毒性效应细胞。CD3阳性、Leu-19阳性淋巴细胞介导针对K562的细胞毒性这一发现明确表明，在未致敏的正常个体外周血中存在非MHC限制的细胞毒性CD3阳性T淋巴细胞的一个独特亚群。然而，CD3阳性、CD16阴性、Leu-19阳性细胞占外周血淋巴细胞不到5%，且该亚群的细胞毒性活性明显低于CD3阴性、CD16阳性、Leu-19阳性NK细胞。大多数CD3阳性、Leu-19阳性T细胞共表达CD2、CD8和CD5分化抗原。外周血CD3阳性、Leu-19阳性细胞毒性T淋巴细胞的抗原性和功能表型与介导针对NK敏感肿瘤细胞靶标的非MHC限制细胞毒性的白细胞介素2依赖性CD3阳性细胞系相对应。还观察到一小群既缺乏CD3又缺乏CD16的Leu-19高表达阳性淋巴细胞。该群体（占外周血淋巴细胞不到2%）包含大无颗粒淋巴细胞和大颗粒淋巴细胞。CD3阴性、CD16阴性、Leu-19高表达阳性淋巴细胞也介导非MHC限制的细胞毒性。这些CD3阴性、CD16阴性、Leu-19高表达阳性淋巴细胞与CD3阳性T细胞或CD16阳性NK细胞的关系尚不清楚。
