Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, University of Tabuk, Tabuk, Saudi Arabia.
Deanship of Academic Affairs, University of Tabuk, Tabuk, Saudi Arabia.
Infect Disord Drug Targets. 2020;20(4):495-500. doi: 10.2174/1871526519666190311165257.
Rapid diagnosis of M. tuberculosis directly from sputum samples is a challenging process. This study aimed to design and evaluate a multiplex-PCR method for direct diagnosis of M. tuberculosis from sputum specimens.
46 suspected tuberculosis patients and 25 apparently healthy individuals were enrolled in the study. Sputa were collected from the study population and processed by cold ZN stain. DNA was extracted from each sample and processed by Multiplex PCR and Genotype Mycobacteria CM.
Out of the 46 Tuberculosis suspected patients, 22 (47.8%) revealed positive Acid fast ba- cilli (AFB), while 19 (41.3%) showed positive by both multiplex PCR and Genotype Mycobacte- ria CM. The overall sensitivity of multiplex PCR and smear microscopy were 100% while the specificity were 100, and 86.3%, respectively.
Multiplex PCR method using two different sets of primers in combination with other diagnostic tools such as X-Rays and smear Microscopy are cheap, rapid and reliable methods for the diagnosis of M. tuberculosis from clinical samples and are able to identify most of the smear positive cases with valuable accuracy.
从痰液样本中快速诊断结核分枝杆菌是一个具有挑战性的过程。本研究旨在设计和评估一种用于直接从痰液标本中诊断结核分枝杆菌的多重 PCR 方法。
本研究纳入了 46 例疑似肺结核患者和 25 名健康个体。从研究人群中采集痰液，并进行冷 ZN 染色。从每个样本中提取 DNA，并通过多重 PCR 和基因型分枝杆菌 CM 进行处理。
在 46 例疑似结核病患者中，22 例（47.8%）经酸染色法（AFB）呈阳性，19 例（41.3%）经多重 PCR 和基因型分枝杆菌 CM 均呈阳性。多重 PCR 和涂片显微镜检查的总灵敏度均为 100%，特异性分别为 100%和 86.3%。
使用两种不同引物组合的多重 PCR 方法与 X 射线和涂片显微镜等其他诊断工具相结合，是一种廉价、快速和可靠的方法，可从临床样本中诊断结核分枝杆菌，并能以有价值的准确性识别大多数涂片阳性病例。
