在小鼠模型中，致病性溶组织内阿米巴和非致病性莫氏内阿米巴的宿主-抗体诱导性。
Host-antibody inductivity of virulent Entamoeba histolytica and non-virulent Entamoeba moshkovskii in a mouse model.
机构信息
Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi, Bangkok, 10400, Thailand.
Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhorn-Pathom, 73170, Thailand.
出版信息
Parasit Vectors. 2019 Mar 13;12(1):101. doi: 10.1186/s13071-019-3363-5.
BACKGROUND
Despite similarities in morphology, gene and protein profiles, Entamoeba histolytica and E. moshkovskii show profound differences in pathogenicity. Entamoeba histolytica infection might result in amoebic dysentery and liver abscess, while E. moshkovskii causes only mild diarrhea. Extensive studies focus on roles of host immune responses to the pathogenic E. histolytica; however, evidence for E. moshkovskii remains scarce.
METHODS
To study differences in host-antibody response profiles between E. histolytica and E. moshkovskii, mice were immunized intraperitoneally with different sets of Entamoeba trophozoites as single species, mixed species and combinations.
RESULTS
Mice prime-immunized with E. histolytica and E. moshkovskii combination, followed by individual species, exhibited higher IgG level than the single species immunization. Mice immunized with E. moshkovskii induced significantly higher levels and long-lasting antibody responses than those challenged with E. histolytica alone. Interestingly, E. histolytica-specific anti-sera promoted the cytopathic ability of E. histolytica toward Chinese hamster ovarian (CHO) cells, but showed no effect on cell adhesion. There was no significant effect of immunized sera on cytopathic activity and adhesion of E. moshkovskii toward both CHO and human epithelial human colonic (Caco-2) cell lines. Monoclonal-antibody (mAb) characterization demonstrated that 89% of E. histolytica-specific mAbs produced from mice targeted cytoplasmic and cytoskeletal proteins, whereas 73% of E. moshkovskii-specific mAbs targeted plasma membrane proteins.
CONCLUSIONS
The present findings suggest that infection with mixed Entamoeba species or E. moshkovskii effectively induces an antibody response in mice. It also sheds light on roles of host antibody response in the pathogenic difference of E. histolytica and E. moshkovskii trophozoites, and cell surface protein modifications of the amoebic parasites to escape from host immune system.
背景
尽管形态学、基因和蛋白质谱相似，但溶组织内阿米巴和穆氏内阿米巴在致病性上存在显著差异。溶组织内阿米巴感染可能导致阿米巴痢疾和肝脓肿，而穆氏内阿米巴仅引起轻度腹泻。广泛的研究集中在宿主免疫反应对致病性溶组织内阿米巴的作用上；然而，关于穆氏内阿米巴的证据仍然很少。
方法
为了研究溶组织内阿米巴和穆氏内阿米巴之间宿主抗体反应谱的差异，用不同的内阿米巴滋养体作为单一物种、混合物种和组合物经腹腔免疫小鼠。
结果
用溶组织内阿米巴和穆氏内阿米巴混合物进行初次免疫，然后用单一物种进行免疫的小鼠，其 IgG 水平高于单一物种免疫。用穆氏内阿米巴免疫的小鼠诱导的抗体水平和持续时间均显著高于单独用溶组织内阿米巴攻击的小鼠。有趣的是，溶组织内阿米巴特异性抗血清促进了溶组织内阿米巴对中国仓鼠卵巢（CHO）细胞的细胞病变能力，但对细胞黏附没有影响。免疫血清对 CHO 和人结肠上皮（Caco-2）细胞系中穆氏内阿米巴的细胞病变活性和黏附没有显著影响。单克隆抗体（mAb）特性表明，从用混合内阿米巴感染的小鼠产生的 89%的溶组织内阿米巴特异性 mAb 针对细胞质和细胞骨架蛋白，而 73%的穆氏内阿米巴特异性 mAb 针对质膜蛋白。
结论
本研究结果表明，混合内阿米巴物种或穆氏内阿米巴感染能有效诱导小鼠产生抗体反应。这也揭示了宿主抗体反应在溶组织内阿米巴和穆氏内阿米巴滋养体致病性差异以及逃避宿主免疫系统的阿米巴寄生虫表面蛋白修饰中的作用。
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