基于DNAzyme和数字PCR的铅的超灵敏检测
Ultrasensitive Detection of Pb Based on a DNAzyme and Digital PCR.
作者信息
Zhang Tao, Liu Cong, Zhou Wuping, Jiang Keming, Yin Chenyu, Liu Cong, Zhang Zhiqiang, Li Haiwen
机构信息
Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
School of Engineering Science, University of Science and Technology, Hefei, China.
出版信息
J Anal Methods Chem. 2019 Jan 2;2019:3528345. doi: 10.1155/2019/3528345. eCollection 2019.
In this study, an ultrasensitive detection method for aqueous Pb based on digital polymerase chain reaction (dPCR) technology and a Pb-dependent DNAzyme was developed. In the presence of Pb, the Gr-5 DNAzyme was activated and catalyzed the hydrolytic cleavage of the substrate strand, resulting in an increase in the amount of template DNA available for dPCR and a resultant change in the number of droplets showing a positive signal. Moreover, the detection system was found to be sensitive and stable in environmental sample detection. In summary, an ultrasensitive quantitative detection method for Pb within environmental substrates was established.
在本研究中，基于数字聚合酶链反应（dPCR）技术和铅依赖性脱氧核酶开发了一种用于检测水溶液中铅的超灵敏检测方法。在铅存在的情况下，Gr-5脱氧核酶被激活并催化底物链的水解切割，导致可用于dPCR的模板DNA量增加，进而使显示阳性信号的液滴数量发生变化。此外，该检测系统在环境样品检测中具有灵敏性和稳定性。总之，建立了一种用于环境基质中铅的超灵敏定量检测方法。
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