Kubelka C F, Ruppel A, Krammer P H, Gemsa D
Parasitology. 1986 Apr;92 ( Pt 2):325-36. doi: 10.1017/s003118200006409x.
The induction of schistosomulicidal activity of peritoneal macrophages by concanavalin A-stimulated supernatants from long-term T-cell clones and by interferon-gamma (IFN-gamma) was investigated in detail. Optimal conditions of in vitro macrophage activation by T-cell clone supernatants were established. Macrophages from 13-week S. mansoni-infected mice responded to lymphokine activation as well as resident macrophages from uninfected mice. IFN-gamma was shown to play an essential role in induction of schistosomulicidal macrophage activity: recombinant IFN-gamma at high concentration could induce schistosomula killing, and an anti-IFN-gamma antiserum inhibited the induction of schistosomulicidal activity by T-cell clone supernatants. Our data also indicate that macrophage activation could be obtained by IFN-gamma in synergy with other lymphokines in the supernatant of long-term T-cell clones. Macrophages from mice injected with T-cell clone supernatants were primed in vivo and triggered to kill schistosomula in vitro in the presence of lipopolysaccharide (LPS). The data demonstrate that lymphokines produced by T-cell clones and, in particular, IFN-gamma can participate in the activation of schistosomulicidal macrophages.
详细研究了来自长期T细胞克隆的伴刀豆球蛋白A刺激上清液和干扰素-γ（IFN-γ）对腹膜巨噬细胞杀血吸虫活性的诱导作用。确定了T细胞克隆上清液体外激活巨噬细胞的最佳条件。感染曼氏血吸虫13周的小鼠的巨噬细胞对淋巴因子激活的反应与未感染小鼠的常驻巨噬细胞相同。结果表明，IFN-γ在诱导杀血吸虫巨噬细胞活性中起重要作用：高浓度的重组IFN-γ可诱导杀灭血吸虫幼虫，抗IFN-γ抗血清可抑制T细胞克隆上清液诱导的杀血吸虫活性。我们的数据还表明，IFN-γ与长期T细胞克隆上清液中的其他淋巴因子协同作用可激活巨噬细胞。注射T细胞克隆上清液的小鼠的巨噬细胞在体内被致敏，并在脂多糖（LPS）存在的情况下在体外被触发杀灭血吸虫幼虫。数据表明，T细胞克隆产生的淋巴因子，特别是IFN-γ，可参与杀血吸虫巨噬细胞的激活。
