Krammer P H, Kubelka C F, Falk W, Ruppel A
J Immunol. 1985 Nov;135(5):3258-63.
We describe a new lymphokine activity, macrophage cytotoxicity inducing factor 2 (MCIF2), in the T cell mitogen-induced supernatant of a murine T cell clone in long-term culture. MCIF2 has the following properties: it elutes from a Sephadex G-100 column in three m.w. forms (10, 34, and 100 KD); it is acid labile (pH 2 to 4) and heat sensitive (80 min at 56 degrees C); it is not constitutively secreted, coexists in the same supernatant with immune interferon (IFN-gamma), and synergizes with IFN-gamma for induction of tumoricidal and schistosomulicidal resident peritoneal mouse macrophages. We uncoupled this synergy and show that IFN-gamma serves as the first ("priming") and MCIF2 as the second ("triggering") signal for macrophage activation. Application of the lymphokines in the reverse order was ineffective. These data demonstrate a two-step mechanism of macrophage activation.
我们描述了一种新的淋巴因子活性，即巨噬细胞细胞毒性诱导因子2（MCIF2），它存在于长期培养的小鼠T细胞克隆经T细胞有丝分裂原诱导后的上清液中。MCIF2具有以下特性：它以三种分子量形式（10、34和100千道尔顿）从Sephadex G - 100柱上洗脱下来；它对酸不稳定（pH 2至4）且对热敏感（56℃下80分钟）；它不是组成性分泌的，与免疫干扰素（IFN - γ）共存于同一上清液中，并与IFN - γ协同作用以诱导驻留腹膜的小鼠巨噬细胞产生杀肿瘤和杀血吸虫幼虫的活性。我们将这种协同作用分开，表明IFN - γ作为巨噬细胞激活的第一个（“启动”）信号，而MCIF2作为第二个（“触发”）信号。以相反顺序应用这些淋巴因子是无效的。这些数据证明了巨噬细胞激活的两步机制。
