Department of Chemistry, Nanchang University, Nanchang, 330031, People's Republic of China.
Mikrochim Acta. 2019 Mar 13;186(4):241. doi: 10.1007/s00604-019-3288-x.
The activity of terminal deoxynucleotidyl transferase (TdTase) is a biomarker for routine diagnosis of acute leukemia. A method has been developed for the determination of TdTase activity. It is based on the use of silver nanoclusters (AgNCs) whose yellow fluorescence is enhanced by an in-situ grown DNA tail of TdTase-polymerized and guanine-rich DNA at the 3' end of a hairpin DNA. The fluorescence, best measured at excitation/emission peaks of 530/585 nm, increases linearly in the 1 to 35 mU mL TdTase activity range. The detection limit is 0.8 mU mL. The method is cost-efficient, selective and convenient. It integrates enhancement of the fluorescence of AgNCs and target recognition into a single process. Graphical abstract Schematic presentation of a method for determination of TdTase activity. It is based on AgNCs fluorescence enhanced by in-situ grown TdTase-polymerized G-rich DNA tail. The method integrates AgNCs fluorescence enhancement and the target recognition into a single process.
端粒酶（TdTase）的活性是常规诊断急性白血病的生物标志物。现已开发出一种用于测定 TdTase 活性的方法。该方法基于使用银纳米簇（AgNCs），其黄色荧光通过 TdTase 聚合和富含鸟嘌呤的 DNA 在发夹 DNA 3'末端原位生长的 DNA 尾巴增强。荧光在激发/发射峰 530/585nm 处最佳测量，在 1 至 35mU mL TdTase 活性范围内呈线性增加。检测限为 0.8mU mL。该方法具有成本效益、选择性和便利性。它将 AgNCs 荧光增强和靶标识别集成到一个单一的过程中。
