克氏锥虫表面、细胞外和核亚蛋白质组的样品制备

Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes.

作者信息

Ricart Carlos André Ornelas, Queiroz Rayner Myr Lauterjung, Dos Santos Júnior Agenor de Castro Moreira, Charneau Sébastien

机构信息

Department of Cell Biology, Laboratory of Protein Chemistry and Biochemistry, University of Brasilia, Brasília, DF, Brazil.

Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge, Cambridge, UK.

出版信息

Methods Mol Biol. 2019;1955:77-87. doi: 10.1007/978-1-4939-9148-8_6.

DOI:10.1007/978-1-4939-9148-8_6

PMID:30868520

Abstract

The preparation of subproteome fractions prior to proteome analysis provides both the enrichment of proteins sub-represented in global proteome analysis and information on the cellular localization of the identified proteins. Here we describe protocols for the preparation of Trypanosoma cruzi surface and extracellular and nuclear fractions for further subproteome analysis.

摘要

在进行蛋白质组分析之前制备亚蛋白质组级分，既能富集在整体蛋白质组分析中含量较低的蛋白质，又能提供有关已鉴定蛋白质细胞定位的信息。在此，我们描述了制备克氏锥虫表面、细胞外和细胞核级分以进行进一步亚蛋白质组分析的实验方案。