Ricci Dante P, Silhavy Thomas J
Department of Early Research, Achaogen, Inc., South San Francisco, CA 94080.
Department of Molecular Biology, Princeton University, Princeton, NJ 08544.
EcoSal Plus. 2019 Mar;8(2). doi: 10.1128/ecosalplus.ESP-0035-2018.
Like all outer membrane (OM) constituents, integral OM β-barrel proteins in Gram-negative bacteria are synthesized in the cytoplasm and trafficked to the OM, where they are locally assembled into the growing OM by the ubiquitous β-barrel assembly machine (Bam). While the identities and structures of all essential and accessory Bam components have been determined, the basic mechanism of Bam-assisted OM protein integration remains elusive. Here we review mechanistic analyses of OM β-barrel protein folding and Bam dynamics and summarize recent insights that inform a general model for OM protein recognition and assembly by the Bam complex.
与所有外膜（OM）成分一样，革兰氏阴性菌中的外膜整合β-桶状蛋白在细胞质中合成，然后运输到外膜，在那里它们通过普遍存在的β-桶状组装机器（Bam）局部组装到不断生长的外膜中。虽然所有必需和辅助Bam成分的身份和结构已被确定，但Bam辅助外膜蛋白整合的基本机制仍然难以捉摸。在这里，我们回顾了外膜β-桶状蛋白折叠和Bam动力学的机制分析，并总结了最近的见解，这些见解为Bam复合物识别和组装外膜蛋白的通用模型提供了依据。
