SVKM's Shri C. B. Patel Research Centre, Vile Parle, Mumbai, Maharashtra 400056, India.
Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai 400094, India and Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Chem Commun (Camb). 2019 Apr 7;55(27):3907-3910. doi: 10.1039/c9cc01262a. Epub 2019 Mar 14.
A fluorescence turn-on probe for amyloid detection in the extreme near-infrared region (>750 nm) is a highly desirable technological evolution from the view point of potential in vivo applications. Herein, we report a molecular rotor-based amyloid sensor probe which, on binding to the insulin amyloid fibril, registers a large turn-on emission in the near-infrared region, and records an exceptionally large red-shifted emission wavelength of ∼770 nm along with a Stokes' shift of ∼150 nm, the highest reported to date for any amyloid sensor probe, in the insulin fibril bound form, in the near-infrared region. Importantly, when bound to insulin fibrils, this probe also exhibits an exceptionally large red-shift of ∼120 nm in the absorption spectra, which enables the naked eye in vitro detection of amyloid fibrils.
一种用于在极端近红外区域（>750nm）检测淀粉样蛋白的荧光开启探针，从潜在的体内应用角度来看，这是一个非常理想的技术发展。在此，我们报告了一种基于分子转子的淀粉样蛋白传感器探针，它与胰岛素淀粉样纤维结合时，在近红外区域显示出大的开启发射，并记录到异常大的红移发射波长约 770nm，以及斯托克斯位移约 150nm，这是迄今为止在近红外区域报道的任何淀粉样蛋白传感器探针的最高值。重要的是，当与胰岛素纤维结合时，该探针在吸收光谱中也表现出异常大的约 120nm 的红移，这使得体外肉眼即可检测淀粉样纤维。
