Department of Chemistry, Faculty of Science, Ilam University, Ilam, Iran.
Department of Chemistry, Faculty of Science, Ilam University, Ilam, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 May 15;215:405-409. doi: 10.1016/j.saa.2019.02.060. Epub 2019 Feb 19.
In this work, salting out liquid-liquid extraction (SALLE) combined with dispersive liquid-liquid microextraction (DLLME) was developed as a novel extraction method for extraction and preconcentration of phenol and chlorophenols (CPs) in environmental water samples. The analytes were derivatized with 4-aminoantipyrineand determined by spectrophotometry. Experimental parameters such as type and volume of the organic solvent, type and amount of salt, pH and vortex time were optimized. Under the optimum conditions, calibration curves were linear in the range of 1-300 μg L and limit of detections (LODs) were in the range of 0.15-0.22 μg L. The extraction recoveries and enrichment factors ranged from 94.80% to 106.1% and 78.12 to 82.53, respectively. Repeatability of method based on five replicate measurements of phenols was in the range of 4.8-7.2%. The results obtained in this study showed that the proposed method is simple, rapid and environmentally friendly with high extraction efficiency for preconcentration and determination of phenol and CPs in real samples. The proposed method was also compared with the reference method.
在这项工作中，盐析液-液萃取（SALLE）与分散液-液微萃取（DLLME）相结合，被开发为一种从环境水样中萃取和预浓缩酚和氯酚（CPs）的新型萃取方法。分析物用 4-氨基安替比林衍生，并通过分光光度法测定。优化了实验参数，如有机溶剂的类型和体积、盐的类型和用量、pH 值和涡旋时间。在最佳条件下，校准曲线在 1-300μg/L 的范围内呈线性，检出限（LOD）在 0.15-0.22μg/L 的范围内。萃取回收率和富集因子的范围分别为 94.80%-106.1%和 78.12-82.53。基于酚类物质的五次重复测量，该方法的重复性在 4.8%-7.2%之间。本研究结果表明，该方法简单、快速、环保，具有高萃取效率，可用于实际样品中酚类和 CPs 的预浓缩和测定。该方法还与参考方法进行了比较。
