Department of Pharmacy, the Second Xiangya Hospital, Central South University, Changsha 410011 PR China; Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, PR China.
Department of Pharmacy, the Second Xiangya Hospital, Central South University, Changsha 410011 PR China; Institute of Clinical Pharmacy, Central South University, Changsha 410011, PR China.
J Pharm Biomed Anal. 2019 May 30;169:159-169. doi: 10.1016/j.jpba.2019.03.009. Epub 2019 Mar 6.
Shen-Fu-Shu granule is traditional Chinese herb formula for acute and chronic renal failure. This work was aimed to identify the compounds in Shen-Fu-Shu granule extract and absorbed compounds in rat plasma following oral administration of Shen-Fu-Shu granule.
A high-resolution and high-sensitivity ultra-performance liquid chromatography with electrospray ionization quadrupole time-of-flight tandem mass spectrometry in positive and negative ion mode were established. The methodology of plasma pharmacochemistry was adopted to analyze the herbal components in vivo. A post-acquisition data processing software UNIFI could automatically, rapidly and accurately achieve the qualitative analysis of the chemical components in Shen-Fu-Shu granule extract and the absorbed components (and their metabolites) in rat plasma after oral administration of Shen-Fu-Shu granule.
Combined with fragments and structure information of LC-Q-TOF-MS, a total of 264 compounds were detected and identified in Shen-Fu-Shu granule extract, while 66 absorbed components (including 38 prototype compounds and 28 metabolites) in plasma were discovered or tentatively characterized in rat plasma.
This study offers a systematically applicable approach for rapid screening and identification of absorbed components derived from multi-herb prescription. It is helpful for understanding the material basis of its therapeutic effects and provides useful information for further study of mechanism of action of Shen-Fu-Shu granule.
参附舒颗粒是一种治疗急、慢性肾衰竭的中药复方制剂。本研究旨在鉴定参附舒颗粒提取物中的化合物和大鼠灌胃参附舒颗粒后血浆中的吸收成分。
采用正、负离子模式的高分辨率、高灵敏度超高效液相色谱-电喷雾四极杆飞行时间串联质谱法，建立了体内药代动力学分析方法。采用血浆药代化学方法分析参附舒颗粒提取物中的草药成分和大鼠灌胃参附舒颗粒后血浆中的吸收成分（及其代谢物）。采用 UNIFI 后采集数据处理软件可自动、快速、准确地实现参附舒颗粒提取物和大鼠灌胃参附舒颗粒后血浆中吸收成分（包括原型化合物和代谢物）的化学成分的定性分析。
结合 LC-Q-TOF-MS 的碎片和结构信息，共鉴定出参附舒颗粒提取物中的 264 种化合物，在大鼠血浆中发现或初步鉴定出 66 种吸收成分（包括 38 种原型化合物和 28 种代谢物）。
本研究为多味中药复方制剂的快速筛选和鉴定提供了一种系统适用的方法，有助于了解其治疗作用的物质基础，并为进一步研究参附舒颗粒的作用机制提供了有用信息。
