Biomed Mater. 2019 Apr 15;14(3):035015. doi: 10.1088/1748-605X/ab0fad.
Isolated nanospheres consisting of organically modified hydroxyapatite (ormoHAP), prepared by an electric field-assisted ion double migration process, were embedded in foamed gelatin to form a composite scaffold. Degradation rates have been demonstrated to correlate with the crosslinking degree (40%, 80%) as well as with the mineral content of the scaffolds (0%, 20%, 40%). A human co-culture model of osteoblasts and osteoclasts, derived from bone marrow stromal cells and monocytes, respectively, without external addition of the factors RANKL and M-CSF, was run for up to 42 d in order to characterize the action of the ormoHAP-gelatin scaffolds on the co-culture. Examination was performed by quantitative biochemical methods (DNA, LDH, ALP, TRAP5b), gene expression analysis (ALP, BSP II, RANKL, IL-6, VTNR, CTSK, TRAP, OSCAR, CALCR) and confocal laser scanning microscopy (cell nuclei, actin, CD68, TRAP). Results confirm that ormoHAP embedded in the gelatin matrix enhanced TRAP 5b activity. As a feedback, ALP activity and gene expression of BSP II of osteoblasts increased. Finally, a sequence of cell cross-talk actions is suggested, which can explain the behavior of the formed vital co-culture and moreover the influence of the presence and concentration of ormoHAP.
采用电场辅助离子双迁移工艺制备的由有机改性羟磷灰石（ormoHAP）组成的纳米球被嵌入到泡沫明胶中以形成复合支架。降解速率与交联度（40%，80%）以及支架的矿物质含量（0%，20%，40%）相关。用人骨髓基质细胞和单核细胞分别培养成成骨细胞和破骨细胞的共培养物模型，在没有外部添加 RANKL 和 M-CSF 因子的情况下，持续运行长达 42 天，以研究 ormoHAP-明胶支架对共培养物的作用。通过定量生化方法（DNA、LDH、ALP、TRAP5b）、基因表达分析（ALP、BSP II、RANKL、IL-6、VTNR、CTSK、TRAP、OSCAR、CALCR）和共聚焦激光扫描显微镜（细胞核、肌动蛋白、CD68、TRAP）进行了检查。结果证实，嵌入明胶基质中的 ormoHAP 增强了 TRAP 5b 的活性。作为反馈，成骨细胞的 ALP 活性和 BSP II 的基因表达增加。最后，提出了一系列细胞串扰作用的序列，可以解释形成的有活力的共培养物的行为，而且还可以解释 ormoHAP 的存在和浓度的影响。
