Díaz-Ruano Susana, López-Pérez Ana E, Girón Rocío, Pérez-García Irene, Martín-Fontelles María I, Abalo Raquel
Unidad de Dolor, Servicio de Anestesiología, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Grupo de Excelencia Investigadora URJC-Banco de Santander-Grupo Multidisciplinar de Investigación y Tratamiento del Dolor (i+DOL), Madrid, Spain.
J Neurogastroenterol Motil. 2019 Apr 30;25(2):300-315. doi: 10.5056/jnm18202.
BACKGROUND/AIMS: Gastrointestinal adverse effects have a major impact on health and quality of life in analgesics users. Non-invasive methods to study gastrointestinal motility are of high interest. Fluoroscopy has been previously used to study gastrointestinal motility in small experimental animals, but they were generally anesthetized and anesthesia itself may alter motility. In this study, our aim is to determine, in conscious rats, the effect of increasing doses of 2 opioid (morphine and loperamide) and 1 cannabinoid (WIN 55,212-2) agonists on colonic motility using fluoroscopic recordings and spatio-temporal maps.
Male Wistar rats received barium sulfate intragastrically, 20-22 hours before fluoroscopy, so that stained fecal pellets could be seen at the time of recording. Animals received an intraperitoneal administration of morphine, loperamide, or WIN 55,212-2 (at 0.1, 1, 5, or 10 mg/kg) or their corresponding vehicles (saline, Cremophor, and Tocrisolve, respectively), 30 minutes before fluoroscopy. Rats were conscious and placed within movement-restrainers for the length of fluoroscopic recordings (120 seconds). Spatio-temporal maps were built, and different parameters were analyzed from the fluoroscopic recordings in a blinded fashion to evaluate colonic propulsion of endogenous fecal pellets.
The analgesic drugs inhibited propulsion of endogenous fecal pellets in a dose-dependent manner.
Fluoroscopy allows studying colonic propulsion of endogenous fecal pellets in conscious rats. Our method may be applied to the non-invasive study of the effect of different drug treatments and pathologies.
背景/目的：胃肠道不良反应对镇痛药使用者的健康和生活质量有重大影响。研究胃肠动力的非侵入性方法备受关注。荧光透视法此前已用于研究小型实验动物的胃肠动力，但这些动物通常处于麻醉状态，而麻醉本身可能会改变胃肠动力。在本研究中，我们的目的是通过荧光透视记录和时空图，确定在清醒大鼠中，增加剂量的2种阿片类药物（吗啡和洛哌丁胺）和1种大麻素（WIN 55,212-2）激动剂对结肠动力的影响。
雄性Wistar大鼠在荧光透视前20 - 22小时经胃内给予硫酸钡，以便在记录时能看到染色的粪便颗粒。在荧光透视前30分钟，动物腹腔注射吗啡、洛哌丁胺或WIN 55,212-2（剂量为0.1、1、5或10 mg/kg）或其相应的赋形剂（分别为生理盐水、聚氧乙烯蓖麻油和Tocrisolve）。大鼠处于清醒状态，在荧光透视记录期间（120秒）置于活动限制器内。构建时空图，并以盲法从荧光透视记录中分析不同参数，以评估内源性粪便颗粒的结肠推进情况。
镇痛药以剂量依赖性方式抑制内源性粪便颗粒的推进。
荧光透视法可用于研究清醒大鼠内源性粪便颗粒的结肠推进情况。我们的方法可应用于不同药物治疗和病理状态影响的非侵入性研究。
