1 Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Ying Wang and Yue Zhou are cofirst authors.
Am J Rhinol Allergy. 2019 Mar;33(2):145-152. doi: 10.1177/1945892418817221.
Predominantly, 2 animal models are used for allergic rhinitis (AR), which are established by intraperitoneal (IP) injection plus local challenge and nasal-only delivery. The differences between these 2 models are not fully understood. Moreover, dose-response relationship to allergens remains unclear.
In this study, mice were sensitized by nasal drops (without adjuvant, once daily for 9 weeks) to set up a nasal-only delivery AR model. Five different doses of ovalbumin (OVA) nasal drops were served to explore the dose-response to allergens. Allergic symptoms, serum antibodies (IgE, IgG2a, and IgG1), spleen supernatant and nasal lavage fluid (NALF) cytokines (IL-4, IL-5, and IFN-r), and infiltrated eosinophils of the nasal mucosa were observed.
The allergic symptoms, serum antibodies, cytokines, and infiltrated eosinophils were significantly higher in the high OVA concentration compared with those of the control group. Different OVA concentrations associated with the severity of allergy. Within a certain concentration range, OVA concentration positively related to the severity of symptoms, IgE antibody level, and Th2 bias. Meanwhile, serum antibodies (IgE and IgG1) and cytokines (IL-4, IL-5 in spleen and IL-4 in NALF) were significantly higher in the classical IP injection group than in the nasal drip groups.
The IP injection model and the nasal-only delivery model are 2 typical models for AR that causes a different immune response. A positive dose-response relationship in the nasal-only delivery model is observed from 25 mg/mL to 0.025 mg/mL.
过敏性鼻炎（AR）主要使用 2 种动物模型，即腹腔（IP）注射加局部挑战和仅鼻腔给药。这两种模型之间的差异尚未完全了解。此外，过敏原的剂量反应关系尚不清楚。
在这项研究中，通过鼻腔滴注（无佐剂，每天 1 次，持续 9 周）使小鼠致敏，以建立仅鼻腔给药 AR 模型。使用 5 种不同剂量的卵清蛋白（OVA）鼻腔滴注液来探索过敏原的剂量反应。观察过敏症状、血清抗体（IgE、IgG2a 和 IgG1）、脾上清液和鼻腔灌洗液（NALF）细胞因子（IL-4、IL-5 和 IFN-r）以及鼻黏膜浸润的嗜酸性粒细胞。
与对照组相比，高 OVA 浓度组的过敏症状、血清抗体、细胞因子和浸润的嗜酸性粒细胞明显更高。不同的 OVA 浓度与过敏的严重程度相关。在一定浓度范围内，OVA 浓度与症状严重程度、IgE 抗体水平和 Th2 偏倚呈正相关。同时，血清抗体（IgE 和 IgG1）和细胞因子（脾中的 IL-4、IL-5 和 NALF 中的 IL-4）在经典 IP 注射组中明显高于鼻腔滴注组。
IP 注射模型和仅鼻腔给药模型是引起不同免疫反应的两种典型 AR 模型。仅鼻腔给药模型中观察到从 25mg/ml 到 0.025mg/ml 的阳性剂量反应关系。
