Schlondorff D, Goldwasser P, Neuwirth R, Satriano J A, Clay K L
Am J Physiol. 1986 Jun;250(6 Pt 2):F1123-7. doi: 10.1152/ajprenal.1986.250.6.F1123.
Platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) results in contraction of isolated glomeruli and cultured mesangial cells and concomitantly causes release of arachidonic acid and prostaglandin E2 (PGE2) formation. The kidney and isolated glomeruli can also generate material that has PAF bioactivity. We therefore examined the capacity of isolated renal glomeruli and cultured glomerular mesangial cells from rats to form PAF. Both isolated glomeruli and cultured mesangial cells transformed 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine ([3H]lyso-PAF) into a labeled product comigrating both on thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC) with authentic PAF. Using rabbit platelet aggregation as bioassay for PAF, we found that isolated glomeruli produced 4 +/- 2 pmol/mg glomerular protein of PAF-like material, and mesangial cells produced 30 +/- 8 pmol/mg cell protein when stimulated with A23187 (10(-5) M) for 30 min. The major species of the PAF material produced by mesangial cells was identified as 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine after HPLC separation, followed by fast atom bombardment and gas chromatography-mass spectrometry. These results show that glomerular mesangial cells can produce PAF, which could contribute locally to the regulation of glomerular function.
血小板活化因子（PAF；1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱）可导致离体肾小球和培养的系膜细胞收缩，并同时引起花生四烯酸的释放和前列腺素E2（PGE2）的形成。肾脏和离体肾小球也能产生具有PAF生物活性的物质。因此，我们研究了大鼠离体肾小球和培养的肾小球系膜细胞形成PAF的能力。离体肾小球和培养的系膜细胞均能将1-O-烷基-2-溶血-sn-甘油-3-磷酸胆碱（[3H]溶血-PAF）转化为一种标记产物，该产物在薄层色谱（TLC）和高压液相色谱（HPLC）上与 authentic PAF 共迁移。以兔血小板聚集作为PAF的生物测定方法，我们发现，用A23187（10^(-5) M）刺激30分钟后，离体肾小球产生4±2 pmol/mg肾小球蛋白的PAF样物质，系膜细胞产生30±8 pmol/mg细胞蛋白。经HPLC分离，随后进行快速原子轰击和气相色谱-质谱分析，确定系膜细胞产生的PAF物质的主要种类为1-O-十六烷基-2-乙酰基-sn-甘油-3-磷酸胆碱。这些结果表明，肾小球系膜细胞能够产生PAF，这可能在局部对肾小球功能的调节起作用。
