Walker B R, Erickson A L, Arnold P E, Burke T J, Berl T
Am J Physiol. 1986 Jun;250(6 Pt 2):R1028-33. doi: 10.1152/ajpregu.1986.250.6.R1028.
Both in vivo as well as in vitro experiments suggest that prostaglandins (PG) may influence arginine vasopressin (AVP) release. Recent studies on conscious dogs have shown that cyclooxygenase inhibition with meclofenamate reduces basal AVP release as well as AVP release in response to hypoxia. The current experiments were performed in order to test whether PG synthesis inhibition affects osmotic- and nonosmotic-stimulated AVP release in a similar manner. Osmotic AVP release was tested by slowly infusing hypertonic saline intravenously in water-diuresing dogs and serially sampling plasma for AVP concentration. Experiments were performed both with and without meclofenamate (2 mg/kg and 2 mg X kg-1 X h-1 iv) pretreatment. AVP release to a comparable osmotic stimulus was greatly reduced after meclofenamate administration. Nonosmotic AVP release was tested by inducing systemic hypotension with an intravenous infusion of nitroprusside. Hypotension was associated with an increase in AVP concentration, which was partially blunted after meclofenamate administration. Experiments performed with only a saline vehicle administered showed no decrease in AVP release in response to comparable hypotension. The findings of these studies suggest that endogenous PG may be involved in both osmotic and nonosmotic AVP release in the conscious dog.
体内和体外实验均表明，前列腺素（PG）可能会影响精氨酸加压素（AVP）的释放。近期针对清醒犬的研究显示，用甲氯芬那酸抑制环氧化酶可降低基础AVP释放以及低氧刺激下的AVP释放。进行当前实验是为了检验PG合成抑制是否以类似方式影响渗透压和非渗透压刺激的AVP释放。通过向处于水利尿状态的犬静脉缓慢输注高渗盐水并连续采集血浆检测AVP浓度，来测试渗透压刺激下的AVP释放。实验分别在给予和未给予甲氯芬那酸（2mg/kg和2mg·kg⁻¹·h⁻¹静脉注射）预处理的情况下进行。给予甲氯芬那酸后，对同等渗透压刺激的AVP释放大幅减少。通过静脉输注硝普钠诱导系统性低血压来测试非渗透压刺激的AVP释放。低血压与AVP浓度升高相关，给予甲氯芬那酸后这种升高有所减弱。仅给予生理盐水载体的实验显示，对同等低血压刺激的AVP释放未减少。这些研究结果表明，内源性PG可能参与清醒犬的渗透压和非渗透压AVP释放过程。
