Calderón Claudia E, Tienda Sandra, Heredia-Ponce Zaira, Arrebola Eva, Cárcamo-Oyarce Gerardo, Eberl Leo, Cazorla Francisco M
Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora," Consejo Superior de Investigaciones Científicas, Universidad de Málaga, IHSM-UMA-CSIC, Málaga, Spain.
Front Microbiol. 2019 Feb 28;10:396. doi: 10.3389/fmicb.2019.00396. eCollection 2019.
The production of the compound 2-hexyl-5-propyl resorcinol (HPR) by the biocontrol rhizobacterium PCL1606 (PcPCL1606) is crucial for fungal antagonism and biocontrol activity that protects plants against the phytopathogenic fungus . The production of HPR is also involved in avocado root colonization during the biocontrol process. This pleiotrophic response prompted us to study the potential role of HPR production in biofilm formation. The swimming motility of PcPLL1606 is enhanced by the disruption of HPR production. Mutants impaired in HPR production, revealed that adhesion, colony morphology, and typical air-liquid interphase pellicles were all dependent on HPR production. The role of HPR production in biofilm architecture was also analyzed in flow chamber experiments. These experiments revealed that the HPR mutant cells had less tight unions than those producing HPR, suggesting an involvement of HPR in the production of the biofilm matrix.
生防根际细菌PCL1606（PcPCL1606）产生的化合物2-己基-5-丙基间苯二酚（HPR）对于真菌拮抗作用和保护植物免受植物病原真菌侵害的生物防治活性至关重要。HPR的产生也参与了生物防治过程中鳄梨根部的定殖。这种多效性反应促使我们研究HPR产生在生物膜形成中的潜在作用。HPR产生的破坏增强了PcPLL1606的游动性。HPR产生受损的突变体表明，粘附、菌落形态和典型的气液界面菌膜均依赖于HPR的产生。还在流动腔实验中分析了HPR产生在生物膜结构中的作用。这些实验表明，HPR突变体细胞的结合不如产生HPR的细胞紧密，这表明HPR参与了生物膜基质的产生。
