尼古丁与一氧化碳：对离体大鼠心脏的影响。

Nicotine and carbon monoxide: effects on the isolated rat heart.

作者信息

McGrath J J

出版信息

Alcohol. 1986 Mar-Apr;3(2):157-60. doi: 10.1016/0741-8329(86)90027-3.

Abstract

We studied the combined effects of carbon monoxide (CO) and nicotine on the performance of the isolated rat heart. Hearts were removed from laboratory rats and perfused through the aorta with 95% O2-5% CO2 (oxygenated) Krebs-Henseleit solution (KHs). After 30 min, the perfusate was switched rapidly to one of three solutions: KHs containing 10% CO-85% O2-5% CO2 (CO), 10 micrograms/ml nicotine (Nic), or CO combined with nicotine (CO + Nic). KHs containing CO increased coronary flow by 41% without affecting heart rate or pulse pressure. Coronary flow returned to control values during recovery in oxygenated KHs. KHs containing Nic decreased heart rate by 20% and coronary flow by 28%. Pulse pressure was unaffected. Both heart rate and coronary flow returned towards control values during recovery in oxygenated KHs. KHs containing CO + Nic decreased heart rate 16% but stimulated coronary flow 13%. Heart rate returned towards control values during recovery; however, coronary flow, which declined to control values after 8 min, continued to decrease during recovery. These results indicate that the separate effects of CO and Nic on coronary flow in the isolated rat heart are opposing and reversible, but that the decrease they produce in combination is irreversible.

摘要

我们研究了一氧化碳（CO）和尼古丁对离体大鼠心脏功能的联合影响。从实验大鼠身上取出心脏，通过主动脉用95% O₂ - 5% CO₂（氧合）的克雷布斯 - 亨泽莱特溶液（KHs）进行灌注。30分钟后，灌注液迅速切换为三种溶液之一：含10% CO - 85% O₂ - 5% CO₂的KHs（CO）、10微克/毫升尼古丁（Nic）或CO与尼古丁的混合物（CO + Nic）。含CO的KHs使冠状动脉血流量增加了41%，而不影响心率或脉压。在氧合KHs中恢复期间，冠状动脉血流量恢复到对照值。含Nic的KHs使心率降低20%，冠状动脉血流量降低28%。脉压未受影响。在氧合KHs中恢复期间，心率和冠状动脉血流量均恢复至对照值。含CO + Nic的KHs使心率降低16%，但刺激冠状动脉血流量增加13%。恢复期间心率恢复至对照值；然而，冠状动脉血流量在8分钟后降至对照值，并在恢复期间持续下降。这些结果表明，CO和Nic对离体大鼠心脏冠状动脉血流量的单独作用是相反且可逆的，但它们联合产生的降低是不可逆的。